Gene expression profile in lprobenfos exposed medaka fish by microarray analysis
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Title
- Gene expression profile in lprobenfos exposed medaka fish by microarray analysis
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Author(s)
- Woo, Seonock; Son, Sung Hee; Ryu, Jae-Chun; Yum, Seungshic
- KIOST Author(s)
- Yum, Seung Shic(염승식)
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Alternative Author(s)
- 손성희; 염승식
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Publication Year
- 2008-06-30
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Abstract
- Differential gene expression profiling was carried out in the hepatic tissue of medaka. fish, Oryzias latipes, after exposure to an organophosphorus pesticide (OPP), lprobenfos (IBP), a widely used pesticide in agri- and fish-culture, using a medaka. cDNA microarray. Twenty six kinds of differentially expressed candidate genes, with 15 and 11 induced and repressed in their gene expressions, respectively, were associated with cytoskeleton (3.8%), development (7.7%), immune (7.7%), metabolism (30.8%), nucleic acid/protein binding (42.3%) and reproduction (7.7%). Of these genes, changes at the transcription level of five were re-evaluated by real-time quantitative PCR (qRT-PCR). Considering the known function of authentic genes, the effects of IBP on the biological activity and pathological aspects in medaka fish were discussed. The identified genes could be used as molecular biomarkers for biological responses to OPPs contamination in an aquatic environment.
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ISSN
- 1738-642X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4489
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Bibliographic Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.2, pp.132 - 137, 2008
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Publisher
- KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
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Subject
- OXIDATIVE STRESS; MESSENGER-RNA; HEPATIC LIPASE; MYTILUS-EDULIS; INDUCTION; TRANSCRIPTION; INHIBITION; BIOMARKERS; ESTERASES; PESTICIDE
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Keywords
- Oryzias latipes; organophosphoruos pesticide (OPP); lprobenfos (IBP); differential gene expression profile; real-time quantitative PCR; microarray
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Type
- Article
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Language
- English
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Document Type
- Article
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