Gene expression profile in lprobenfos exposed medaka fish by microarray analysis SCIE KCI OTHER

Title
Gene expression profile in lprobenfos exposed medaka fish by microarray analysis
Author(s)
Woo, Seonock; Son, Sung Hee; Ryu, Jae-Chun; Yum, Seungshic
KIOST Author(s)
Yum, Seung Shic(염승식)
Publication Year
2008-06-30
Abstract
Differential gene expression profiling was carried out in the hepatic tissue of medaka. fish, Oryzias latipes, after exposure to an organophosphorus pesticide (OPP), lprobenfos (IBP), a widely used pesticide in agri- and fish-culture, using a medaka. cDNA microarray. Twenty six kinds of differentially expressed candidate genes, with 15 and 11 induced and repressed in their gene expressions, respectively, were associated with cytoskeleton (3.8%), development (7.7%), immune (7.7%), metabolism (30.8%), nucleic acid/protein binding (42.3%) and reproduction (7.7%). Of these genes, changes at the transcription level of five were re-evaluated by real-time quantitative PCR (qRT-PCR). Considering the known function of authentic genes, the effects of IBP on the biological activity and pathological aspects in medaka fish were discussed. The identified genes could be used as molecular biomarkers for biological responses to OPPs contamination in an aquatic environment.
ISSN
1738-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4489
Bibliographic Citation
MOLECULAR & CELLULAR TOXICOLOGY, v.4, no.2, pp.132 - 137, 2008
Publisher
KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
Subject
OXIDATIVE STRESS; MESSENGER-RNA; HEPATIC LIPASE; MYTILUS-EDULIS; INDUCTION; TRANSCRIPTION; INHIBITION; BIOMARKERS; ESTERASES; PESTICIDE
Keywords
Oryzias latipes; organophosphoruos pesticide (OPP); lprobenfos (IBP); differential gene expression profile; real-time quantitative PCR; microarray
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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