Screening enantioselective epoxide hydrolase activities from marine microorganisms: Detection of activities in Erythrobacter spp. SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Screening enantioselective epoxide hydrolase activities from marine microorganisms: Detection of activities in Erythrobacter spp.
Author(s)
Hwang, Young-Ok; Kang, Sung Gyun; Woo, Jung-Hee; Kwon, Kye Kyung; Sato, Takako; Lee, Eun Yeol; Han, Myong Soo; Kim, Sang-Jin
KIOST Author(s)
Kang, Sung Gyun(강성균)Kwon, Kae Kyoung(권개경)
Publication Year
2008-07
Abstract
To develop an enantioselective epoxide hydrolase (EHase) from marine microorganisms, marine samples were collected from a variety of marine environments. Strains isolated by the capability of living on styrene oxide (SO) were screened for retaining enantioselective EHase activities toward SO by combining spectrophotometric, GC, and HPLC analysis. Consequently, one strain, JCS358, was selected, and the sequence analysis of 16S rRNA gene showed that the strain belonged to Erythrobacter cluster. Twelve additional Erythrobacter strains from this study or acquired from culture collections were thereby tested for displaying EHase activities, and most of tested strains showed enantioselective hydrolysis toward SO and glycidyl phenyl ether. Kinetic resolution of racemic SO using whole cell of Erythrobacter sp. JCS358 was performed. Enantiopure (S)-SO could be obtained with an enantiomeric excess (ee) higher than 99% after 15 h incubation. The determination of 1-phenyl-1,2-ethanediol configuration derived from racemic SO confirmed the enantioselective hydrolyzing activity of Erythrobacter sp. JCS358.
ISSN
1436-2228
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4488
DOI
10.1007/s10126-007-9070-9
Bibliographic Citation
MARINE BIOTECHNOLOGY, v.10, no.4, pp.366 - 373, 2008
Publisher
SPRINGER
Subject
SP-NOV; GEN-NOV; SPHINGOMONAS; BACTERIOCHLOROPHYLL; SEA; RADIOBACTER; HYDROLYSIS; BACTERIUM; STRAINS
Keywords
screening; epoxide hydrolase; marine microorganism; Erythrobacter
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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