Screening enantioselective epoxide hydrolase activities from marine microorganisms: Detection of activities in Erythrobacter spp.
SCIE
SCOPUS
Cited 16 time in
WEB OF SCIENCE
Cited 18 time in
Scopus
-
Title
- Screening enantioselective epoxide hydrolase activities from marine microorganisms: Detection of activities in Erythrobacter spp.
-
Author(s)
- Hwang, Young-Ok; Kang, Sung Gyun; Woo, Jung-Hee; Kwon, Kye Kyung; Sato, Takako; Lee, Eun Yeol; Han, Myong Soo; Kim, Sang-Jin
- KIOST Author(s)
- Kang, Sung Gyun(강성균); Kwon, Kae Kyoung(권개경)
-
Alternative Author(s)
- 황영옥; 강성균; 우정희; 권개경; 김상진
-
Publication Year
- 2008-07
-
Abstract
- To develop an enantioselective epoxide hydrolase (EHase) from marine microorganisms, marine samples were collected from a variety of marine environments. Strains isolated by the capability of living on styrene oxide (SO) were screened for retaining enantioselective EHase activities toward SO by combining spectrophotometric, GC, and HPLC analysis. Consequently, one strain, JCS358, was selected, and the sequence analysis of 16S rRNA gene showed that the strain belonged to Erythrobacter cluster. Twelve additional Erythrobacter strains from this study or acquired from culture collections were thereby tested for displaying EHase activities, and most of tested strains showed enantioselective hydrolysis toward SO and glycidyl phenyl ether. Kinetic resolution of racemic SO using whole cell of Erythrobacter sp. JCS358 was performed. Enantiopure (S)-SO could be obtained with an enantiomeric excess (ee) higher than 99% after 15 h incubation. The determination of 1-phenyl-1,2-ethanediol configuration derived from racemic SO confirmed the enantioselective hydrolyzing activity of Erythrobacter sp. JCS358.
-
ISSN
- 1436-2228
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4488
-
DOI
- 10.1007/s10126-007-9070-9
-
Bibliographic Citation
- MARINE BIOTECHNOLOGY, v.10, no.4, pp.366 - 373, 2008
-
Publisher
- SPRINGER
-
Subject
- SP-NOV; GEN-NOV; SPHINGOMONAS; BACTERIOCHLOROPHYLL; SEA; RADIOBACTER; HYDROLYSIS; BACTERIUM; STRAINS
-
Keywords
- screening; epoxide hydrolase; marine microorganism; Erythrobacter
-
Type
- Article
-
Language
- English
-
Document Type
- Article
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.