Sensing domain and extension rate of a family B-type DNA polymerase determine the stalling at a deaminated base SCIE SCOPUS KCI

DC Field Value Language
dc.contributor.author Kim, Yun Jae -
dc.contributor.author Cha, Sun-Shin -
dc.contributor.author Lee, Hyun Sook -
dc.contributor.author Ryu, Yong Gu -
dc.contributor.author Bae, Seung Seob -
dc.contributor.author Cho, Yona -
dc.contributor.author Cho, Hyun-Soo -
dc.contributor.author Kim, Sang-Jin -
dc.contributor.author Kwon, Suk-Tae -
dc.contributor.author Lee, Jung-Hyun -
dc.contributor.author Kang, Sung Gyun -
dc.date.accessioned 2020-04-20T10:40:39Z -
dc.date.available 2020-04-20T10:40:39Z -
dc.date.created 2020-01-28 -
dc.date.issued 2008-08 -
dc.identifier.issn 1017-7825 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4476 -
dc.description.abstract The uracil-sensing domain in archaeal family B-type DNA polymerases recognizes pro-mutagenic uracils in the DNA template, leading to stalling of DNA polymerases. Here, we describe our new findings regarding the molecular mechanism underpinning the stalling of polymerases. We observed that two successive deaminated bases were required to stall TNA1 and KOD1 DNA polymerases, whereas a single deaminated base was enough for stalling Pfu DNA polymerase, in spite of the virtually identical uracil-sensing domains. TNA1 and KOD1 DNA polymerases; have a much higher extension rate than Pfu DNA polymerase; decreasing the extension rate resulted in stalling by TNA1 and KOD1 DNA polymerases at a single deaminated base. These results strongly suggest that these polymerases require two factors to stop DNA polymerization at a single deaminated base: the presence of the uracil-sensing domain and a relatively slow extension rate. -
dc.description.uri 1 -
dc.language English -
dc.publisher KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY -
dc.subject THERMOCOCCUS-ONNURINEUS NA1 -
dc.subject HYPERTHERMOPHILIC ARCHAEON -
dc.subject BIOCHEMICAL-CHARACTERIZATION -
dc.subject DEOXYRIBONUCLEIC-ACID -
dc.subject URACIL -
dc.subject PCR -
dc.subject REPLICATION -
dc.subject RECOGNITION -
dc.subject GLYCOSYLASE -
dc.subject INSTABILITY -
dc.title Sensing domain and extension rate of a family B-type DNA polymerase determine the stalling at a deaminated base -
dc.type Article -
dc.citation.endPage 1385 -
dc.citation.startPage 1377 -
dc.citation.title JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY -
dc.citation.volume 18 -
dc.citation.number 8 -
dc.contributor.alternativeName 김윤재 -
dc.contributor.alternativeName 차선신 -
dc.contributor.alternativeName 이현숙 -
dc.contributor.alternativeName 배승섭 -
dc.contributor.alternativeName 김상진 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.8, pp.1377 - 1385 -
dc.identifier.scopusid 2-s2.0-56749103925 -
dc.identifier.wosid 000258787500005 -
dc.type.docType Article -
dc.identifier.kciid ART001274826 -
dc.description.journalClass 1 -
dc.subject.keywordPlus THERMOCOCCUS-ONNURINEUS NA1 -
dc.subject.keywordPlus HYPERTHERMOPHILIC ARCHAEON -
dc.subject.keywordPlus BIOCHEMICAL-CHARACTERIZATION -
dc.subject.keywordPlus DEOXYRIBONUCLEIC-ACID -
dc.subject.keywordPlus URACIL -
dc.subject.keywordPlus PCR -
dc.subject.keywordPlus REPLICATION -
dc.subject.keywordPlus RECOGNITION -
dc.subject.keywordPlus GLYCOSYLASE -
dc.subject.keywordPlus INSTABILITY -
dc.subject.keywordAuthor archaeal family B-type DNA polymerase -
dc.subject.keywordAuthor uracil sensing -
dc.subject.keywordAuthor TNA1 DNA polymerase -
dc.subject.keywordAuthor extension rate -
dc.subject.keywordAuthor hypoxanthine -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalWebOfScienceCategory Microbiology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.description.journalRegisteredClass kci -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Microbiology -
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