Sensing domain and extension rate of a family B-type DNA polymerase determine the stalling at a deaminated base SCIE SCOPUS KCI

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Sensing domain and extension rate of a family B-type DNA polymerase determine the stalling at a deaminated base
Author(s)
Kim, Yun Jae; Cha, Sun-Shin; Lee, Hyun Sook; Ryu, Yong Gu; Bae, Seung Seob; Cho, Yona; Cho, Hyun-Soo; Kim, Sang-Jin; Kwon, Suk-Tae; Lee, Jung-Hyun; Kang, Sung Gyun
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2008-08
Abstract
The uracil-sensing domain in archaeal family B-type DNA polymerases recognizes pro-mutagenic uracils in the DNA template, leading to stalling of DNA polymerases. Here, we describe our new findings regarding the molecular mechanism underpinning the stalling of polymerases. We observed that two successive deaminated bases were required to stall TNA1 and KOD1 DNA polymerases, whereas a single deaminated base was enough for stalling Pfu DNA polymerase, in spite of the virtually identical uracil-sensing domains. TNA1 and KOD1 DNA polymerases; have a much higher extension rate than Pfu DNA polymerase; decreasing the extension rate resulted in stalling by TNA1 and KOD1 DNA polymerases at a single deaminated base. These results strongly suggest that these polymerases require two factors to stop DNA polymerization at a single deaminated base: the presence of the uracil-sensing domain and a relatively slow extension rate.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4476
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.18, no.8, pp.1377 - 1385, 2008
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
THERMOCOCCUS-ONNURINEUS NA1; HYPERTHERMOPHILIC ARCHAEON; BIOCHEMICAL-CHARACTERIZATION; DEOXYRIBONUCLEIC-ACID; URACIL; PCR; REPLICATION; RECOGNITION; GLYCOSYLASE; INSTABILITY
Keywords
archaeal family B-type DNA polymerase; uracil sensing; TNA1 DNA polymerase; extension rate; hypoxanthine
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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