Induction of apoptosis by streptochlorin isolated from Streptomyces sp. in human leukemic U937 cells SCIE SCOPUS

Cited 24 time in WEB OF SCIENCE Cited 24 time in Scopus
Title
Induction of apoptosis by streptochlorin isolated from Streptomyces sp. in human leukemic U937 cells
Author(s)
Park, C.; Shin, H.J.; Kim, G.-Y.; Kwon, T.K.; Nam, T.-J.; Kim, S.-K.; Cheong, J.; Choi, I.-W.; Choi, Y.H.
Publication Year
2008-09
Abstract
Streptochlorin is a small molecule that produced by marine Streptomyces sp. that is known to have anti-angiogenic and anti-cancer properties. However, the mechanism by which streptochlorin functions is not well understood. In this study, we investigated the pro-apoptotic effect of streptochlorin in human leukemic U937 cells. Streptochlorin treatment resulted in concentration- and time-dependent growth inhibition by inducing apoptosis. The increase in apoptosis that was induced by streptochlorin was correlated with down-regulation of anti-apoptotic Bcl-2 expression, up-regulation of pro-apoptotic Bax and FasL, a decrease in the mitochondrial membrane potential (MMP), activation of caspases and degradation of poly-(ADP-ribose)polymerase and phospholipase C-γ1 protein. In addition, the cytotoxic effects and apoptotic characteristics induced by streptochlorin were significantly inhibited by z-DEVD-fmk, a caspase-3 inhibitor, which demonstrates the important role that caspase-3 played in the process. Furthermore, Bcl-2 overexpression significantly reversed the streptochlorin-induced growth inhibitory effects via inhibition of the MMP collapse and caspases activation and effectively attenuated the apoptotic response to streptochlorin. However, the elevated levels of FasL expression induced by streptochlorin were not reduced by Bcl-2 overexpression. Taken together, these findings demonstrate that the pro-apoptotic effect of streptochlorin is mediated through activation of caspases and mitochondria in U937 cells. © 2008 Elsevier Ltd. All rights reserved.
ISSN
0887-2333
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4455
DOI
10.1016/j.tiv.2008.06.010
Bibliographic Citation
Toxicology in Vitro, v.22, no.6, pp.1573 - 1581, 2008
Publisher
Elsevier BV
Keywords
Apoptosis; Bcl-2; Caspase-3; Streptochlorin; U937
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier BV
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse