A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion
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Title
- A novel function of karyopherin beta 3 associated with apolipoprotein A-I secretion
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Author(s)
- Chung, Kyung Min; Cha, Sun-Shin; Jang, Sung Key
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Alternative Author(s)
- 차선신
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Publication Year
- 2008-09-30
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Abstract
- Human karyopherin beta 3, highly homologous to a yeast protein secretion enhancer (PSE1), has often been reported to be associated with a mediator of a nucleocytoplasmic transport pathway. Previously, we showed that karyopherin beta 3 complemented the PSE1 and KAP123 double mutant. Our research suggested that karyopherin beta 3 has an evolutionary function similar to that of yeast PSE1 and/or KAP 123. In this study, we performed yeast two-hybrid screening to find a protein which would interact with karyopherin beta 3 and identified apolipoprotein A-I (apo A-I), a secretion protein with a primary function in cholesterol transport. By using in vitro binding assay, coimmunoprecipitation, and colocalization studies, we defined an interaction between karyopherin beta 3 and apo A-I. In addition, overexpression of karyopherin beta 3 significantly increased apo A-I secretion. These results suggest that karyopherin beta 3 plays a crucial role in apo A-I secretion. These findings may be relevant to the study of a novel function of karyopherin beta 3 and coronary artery diseases associated with apo A-I.
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ISSN
- 1016-8478
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4448
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Bibliographic Citation
- MOLECULES AND CELLS, v.26, no.3, pp.291 - 298, 2008
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Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
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Subject
- NUCLEAR IMPORT RECEPTORS; MINOR CAPSID PROTEIN; ENDOPLASMIC-RETICULUM; SACCHAROMYCES-CEREVISIAE; RIBOSOMAL-PROTEINS; CHOLESTEROL EFFLUX; MAMMALIAN-CELLS; PORE COMPLEX; TRANSPORT; INTERACTS
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Keywords
- apolipoprotein A-I; coronary artery diseases; karyopherin beta 3; PSE1; secretion enhancer
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Type
- Article
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Language
- English
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Document Type
- Article
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