High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Min-Jeong | - |
dc.contributor.author | Kim, Seon-Young | - |
dc.contributor.author | Kim, Ki-Hong | - |
dc.contributor.author | Yoo, Sung-Sik | - |
dc.contributor.author | Lee, Taek Kyun | - |
dc.contributor.author | Choi, Tae-Jin | - |
dc.date.accessioned | 2023-07-16T23:50:02Z | - |
dc.date.available | 2023-07-16T23:50:02Z | - |
dc.date.created | 2023-07-14 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 2076-393X | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/44422 | - |
dc.description.abstract | The demand for aquaculture is increasing, but production is declining due to high feed costs and disease outbreaks. Viral hemorrhagic septicemia (VHS) is a viral disease that seriously affects seawater and freshwater fish in aquaculture, including the olive flounder (Paralichthys olivaceus), a major aquaculture fish in Korea. However, very few vaccines are currently available for viral hemorrhagic septicemia virus (VHSV). The nutrient-rich microalga Chlorella vulgaris has been used as a feed additive in aquaculture and as a host for the industrial production of recombinant VHSV glycoprotein as an oral vaccine. In this study, VHSV glycoprotein was cloned with a salt-inducible promoter, and high levels of expression up to 41.1 mg/g wet C. vulgaris, representing 27.4% of total extracted soluble protein, were achieved by growing the transformed C. vulgaris for 5 days in the presence of 250 mM NaCl. The production of a neutralizing antibody was detected in the serum of fish given feed containing 9% VHSV glycoprotein-expressing C. vulgaris. Furthermore, relative survival rates of 100% and 81.9% were achieved following challenges of these fish with VHSV at 106 and 107 pfu/fish, respectively, indicating that C. vulgaris could be used as a platform for the production of recombinant proteins for use as oral vaccines in the control of viral diseases in aquaculture. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | High-Level Expression of Recombinant VHSV Glycoprotein Using Transformed C. vulgaris and Verification of Vaccine Efficacy | - |
dc.type | Article | - |
dc.citation.title | Vaccines | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | Vaccines, v.11, no.7 | - |
dc.identifier.doi | 10.3390/vaccines11071205 | - |
dc.identifier.scopusid | 2-s2.0-85166419653 | - |
dc.identifier.wosid | 001073294100001 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.subject.keywordPlus | HEMORRHAGIC SEPTICEMIA VIRUS | - |
dc.subject.keywordPlus | FIBROBLAST-GROWTH-FACTOR | - |
dc.subject.keywordPlus | CHLORELLA-VULGARIS | - |
dc.subject.keywordPlus | RAINBOW-TROUT | - |
dc.subject.keywordPlus | FISH VACCINES | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | RHABDOVIRUSES | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | FISHERIES | - |
dc.subject.keywordPlus | FLOUNDER | - |
dc.subject.keywordAuthor | VHSV | - |
dc.subject.keywordAuthor | oral vaccine | - |
dc.subject.keywordAuthor | chlorella | - |
dc.subject.keywordAuthor | microalga | - |
dc.subject.keywordAuthor | salt-inducible promoter | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |