Exosomes from bacteria (Streptococcus parauberis) challenged olive flounder (Paralichthys olivaceus): Isolation, molecular characterization, wound healing, and regeneration activities SCIE SCOPUS

DC Field Value Language
dc.contributor.author Jayathilaka, E.H.T.Thulshan -
dc.contributor.author Edirisinghe, S. L. -
dc.contributor.author Oh, Chul Hong -
dc.contributor.author Nikapitiya, Chamilani -
dc.contributor.author De, Zoysa Mahanama -
dc.date.accessioned 2023-05-08T03:50:00Z -
dc.date.available 2023-05-08T03:50:00Z -
dc.date.created 2023-05-08 -
dc.date.issued 2023-06 -
dc.identifier.issn 1050-4648 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/44227 -
dc.description.abstract Exosomes are a group of extracellular vesicles carrying membrane proteins, lipids, RNAs, and, cytosolic proteins, which play key role in intercellular communication and homeostasis. This study describes the isolation, physicochemical, morphological and molecular characterization, toxicity, wound healing, and regeneration properties of plasma derived exosomes from naive (phosphate-buffered saline [PBS]-injected; PBS-Exo) and Streptococcus parauberis-challenged (Sp-Exo) olive flounder (Paralichthys olivaceus). The average diameters of PBS-Exo and Sp-Exo were 120.5 ± 6.1 and 113.1 ± 9.3 nm, respectively, and they presented unique cup shape morphologies. Both exosomes exhibited classical tetraspanin surface markers (CD81, CD9, and CD63) and were enriched with acetylcholinesterase. High-throughput miRNA profiling revealed differentially expressed miRNAs (log2 fold change ≥1; P < 0.05), including 14 known and 22 novel miRNAs, in Sp-Exo. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that the target genes of the miRNAs contribute towards various physiological and immunological functions, including wound healing and fin regeneration. Sp-Exo exhibited a rapid wound healing (cell migration) capacity in human fibroblast cells, and its mRNA and protein expression patterns corroborated its activity. Higher larval fin regeneration was more prevalent in Sp-Exo than in PBS-Exo, which further confirmed its functional significance. Our study provides the first basic physiochemical, morphometric, molecular (miRNA profiling), and wound healing evidences of Sp-Exo in olive flounder and highlights important miRNA cargoes in exosomes that may be potential therapeutic candidates in wound healing. -
dc.description.uri 1 -
dc.language English -
dc.publisher Academic Press -
dc.title Exosomes from bacteria (Streptococcus parauberis) challenged olive flounder (Paralichthys olivaceus): Isolation, molecular characterization, wound healing, and regeneration activities -
dc.type Article -
dc.citation.title Fish and Shellfish Immunology -
dc.citation.volume 137 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation Fish and Shellfish Immunology, v.137 -
dc.identifier.doi 10.1016/j.fsi.2023.108777 -
dc.identifier.scopusid 2-s2.0-85153928637 -
dc.identifier.wosid 000998665900001 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.description.isOpenAccess N -
dc.subject.keywordPlus MICRORNAS -
dc.subject.keywordPlus PROMOTES -
dc.subject.keywordPlus GENE -
dc.subject.keywordAuthor Cell migration -
dc.subject.keywordAuthor Extracellular vesicles -
dc.subject.keywordAuthor Exosome -
dc.subject.keywordAuthor miRNAs -
dc.subject.keywordAuthor Olive flounder -
dc.subject.keywordAuthor Paralichthys olivaceus -
dc.subject.keywordAuthor Streptococcus parauberis -
dc.subject.keywordAuthor Wound healing -
dc.subject.keywordAuthor Zebrafish -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.relation.journalWebOfScienceCategory Immunology -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.relation.journalWebOfScienceCategory Veterinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Fisheries -
dc.relation.journalResearchArea Immunology -
dc.relation.journalResearchArea Marine & Freshwater Biology -
dc.relation.journalResearchArea Veterinary Sciences -
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Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
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