Development of human serum albumin selective fluorescent probe using thieno[3,2-b]pyridine-5(4h)one fluorophore derivatives SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Lee S. | - |
dc.contributor.author | Sung D.-B. | - |
dc.contributor.author | Kang S. | - |
dc.contributor.author | Parameswaran S. | - |
dc.contributor.author | Choi J.-H. | - |
dc.contributor.author | Lee J.S. | - |
dc.contributor.author | Han M.S. | - |
dc.date.accessioned | 2020-04-16T07:05:14Z | - |
dc.date.available | 2020-04-16T07:05:14Z | - |
dc.date.created | 2020-02-04 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/441 | - |
dc.description.abstract | The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4 in Scheme 1) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Development of human serum albumin selective fluorescent probe using thieno[3,2-b]pyridine-5(4h)one fluorophore derivatives | - |
dc.type | Article | - |
dc.citation.title | Sensors | - |
dc.citation.volume | 19 | - |
dc.citation.number | 23 | - |
dc.contributor.alternativeName | 성단비 | - |
dc.contributor.alternativeName | 이종석 | - |
dc.identifier.bibliographicCitation | Sensors, v.19, no.23 | - |
dc.identifier.doi | 10.3390/s19235298 | - |
dc.identifier.scopusid | 2-s2.0-85076048371 | - |
dc.identifier.wosid | 000507606200241 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordPlus | Charge transfer | - |
dc.subject.keywordPlus | Diagnosis | - |
dc.subject.keywordPlus | Fluorescence | - |
dc.subject.keywordPlus | Mammals | - |
dc.subject.keywordPlus | Probes | - |
dc.subject.keywordPlus | Proteins | - |
dc.subject.keywordPlus | Pyridine | - |
dc.subject.keywordPlus | Fluorescent probes | - |
dc.subject.keywordPlus | Human serum albumins | - |
dc.subject.keywordPlus | Intra-molecular charge transfer | - |
dc.subject.keywordPlus | Microalbuminuria | - |
dc.subject.keywordPlus | Thieno[3, 2-b]pyridine-5(4H)-one | - |
dc.subject.keywordPlus | Fluorophores | - |
dc.subject.keywordAuthor | Fluorescent probe | - |
dc.subject.keywordAuthor | Human serum albumin | - |
dc.subject.keywordAuthor | Intramolecular charge transfer | - |
dc.subject.keywordAuthor | Microalbuminuria | - |
dc.subject.keywordAuthor | Thieno[3, 2-b]pyridine-5(4H)-one | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |