Development of human serum albumin selective fluorescent probe using thieno[3,2-b]pyridine-5(4h)one fluorophore derivatives SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
Development of human serum albumin selective fluorescent probe using thieno[3,2-b]pyridine-5(4h)one fluorophore derivatives
Author(s)
Lee S.; Sung D.-B.; Kang S.; Parameswaran S.; Choi J.-H.; Lee J.S.; Han M.S.
KIOST Author(s)
Sung, Dan Bi(성단비)Lee, Jong Soo(이종수)
Publication Year
2019-12
Abstract
The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4 in Scheme 1) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
ISSN
1424-8220
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/441
DOI
10.3390/s19235298
Bibliographic Citation
Sensors, v.19, no.23, 2019
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Keywords
Fluorescent probe; Human serum albumin; Intramolecular charge transfer; Microalbuminuria; Thieno[3, 2-b]pyridine-5(4H)-one
Type
Article
Language
English
Document Type
Article
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Related Researcher
Research Interests

Total synthesis of natrual product,Synthesis of organic chemistry,천연물 전합성,유기합성

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