Probiotic effects of Pseudoalteromonas ruthenica: Antibacterial, immune stimulation and modulation of gut microbiota composition SCIE SCOPUS

DC Field Value Language
dc.contributor.author Wasana, Withanage Prasadini -
dc.contributor.author Senevirathne, Amal -
dc.contributor.author Nikapitiya, Chamilani -
dc.contributor.author Lee, Jong-Soo -
dc.contributor.author Kang, Do Hyung -
dc.contributor.author Kwon, Kae Kyoung -
dc.contributor.author Oh, Chul Hong -
dc.contributor.author De Zoysa, Mahanama -
dc.date.accessioned 2022-10-17T02:30:01Z -
dc.date.available 2022-10-17T02:30:01Z -
dc.date.created 2022-10-17 -
dc.date.issued 2022-12 -
dc.identifier.issn 1050-4648 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/43268 -
dc.description.abstract This study aimed to characterise and evaluate the probiotic properties of a newly isolated marine bacterium, strain S6031. The isolated strain was identified as Pseudoalteromonas ruthenica. In vivo experiments were conducted with P. ruthenica-immersed larvae and P. ruthenica-enriched Artemia fed to adult zebrafish. Disease tolerance of larval zebrafish against Edwardsiella piscicida was demonstrated by 66.34% cumulative per cent survival (CPS) in the P. ruthenica-exposed group, which was higher than the CPS of the control (46.67%) at 72 h post challenge (hpc). Heat-stressed larvae had 55% CPS in the P. ruthenica-immersed group, while the control had 30% CPS at 60 hpc. Immune-stress response gene transcripts (muc5.1, muc5.2, muc5.3, alpi2, alpi3, hsp70, and hsp90a) were induced, while pro-inflammatory genes (tnfα, il1b, and il6) were downregulated in P. ruthenica-immersed larvae compared to the control. This trend was confirmed by low pro-inflammatory and high stress-responsive protein expression levels in P. ruthenica-exposed larvae. Adult zebrafish had higher CPS (27.2%) in the P. ruthenica-fed group than the control (9.52%) upon E. piscicida challenge, suggesting increased disease tolerance. Histological analysis demonstrated modulation of goblet cell density and average villus height in the P. ruthenica-supplemented group. Metagenomics analysis clearly indicated modulation of alpha diversity indices and the relative abundance of Proteobacteria in the P. ruthenica-supplemented zebrafish gut. Furthermore, increased Firmicutes colonisation and reduced Bacteroidetes abundance in the gut were observed upon P. ruthenica supplementation. Additionally, this study confirmed the concentration-dependent increase of colony dispersion and macrophage uptake upon mucin treatment. In summary, P. ruthenica possesses remarkable functional properties as a probiotic that enhances host defence against diseases and thermal stress. -
dc.description.uri 1 -
dc.language English -
dc.publisher Academic Press -
dc.title Probiotic effects of Pseudoalteromonas ruthenica: Antibacterial, immune stimulation and modulation of gut microbiota composition -
dc.type Article -
dc.citation.endPage 243 -
dc.citation.startPage 229 -
dc.citation.title Fish and Shellfish Immunology -
dc.citation.volume 131 -
dc.contributor.alternativeName 강도형 -
dc.contributor.alternativeName 권개경 -
dc.contributor.alternativeName 오철홍 -
dc.identifier.bibliographicCitation Fish and Shellfish Immunology, v.131, pp.229 - 243 -
dc.identifier.doi 10.1016/j.fsi.2022.09.070 -
dc.identifier.scopusid 2-s2.0-85140036574 -
dc.identifier.wosid 000876610900003 -
dc.description.journalClass 1 -
dc.description.isOpenAccess N -
dc.subject.keywordAuthor Pseudoalteromonas ruthenica -
dc.subject.keywordAuthor Probiotics -
dc.subject.keywordAuthor Bioencapsulation -
dc.subject.keywordAuthor Metagenomics -
dc.subject.keywordAuthor Disease tolerance -
dc.subject.keywordAuthor Zebrafish -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.relation.journalWebOfScienceCategory Immunology -
dc.relation.journalWebOfScienceCategory Marine & Freshwater Biology -
dc.relation.journalWebOfScienceCategory Veterinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
Appears in Collections:
Jeju Research Institute > Jeju Bio Research Center > 1. Journal Articles
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 1. Journal Articles
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