Probiotic effects of Pseudoalteromonas ruthenica: Antibacterial, immune stimulation and modulation of gut microbiota composition SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Wasana, Withanage Prasadini | - |
dc.contributor.author | Senevirathne, Amal | - |
dc.contributor.author | Nikapitiya, Chamilani | - |
dc.contributor.author | Lee, Jong-Soo | - |
dc.contributor.author | Kang, Do Hyung | - |
dc.contributor.author | Kwon, Kae Kyoung | - |
dc.contributor.author | Oh, Chul Hong | - |
dc.contributor.author | De Zoysa, Mahanama | - |
dc.date.accessioned | 2022-10-17T02:30:01Z | - |
dc.date.available | 2022-10-17T02:30:01Z | - |
dc.date.created | 2022-10-17 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/43268 | - |
dc.description.abstract | This study aimed to characterise and evaluate the probiotic properties of a newly isolated marine bacterium, strain S6031. The isolated strain was identified as Pseudoalteromonas ruthenica. In vivo experiments were conducted with P. ruthenica-immersed larvae and P. ruthenica-enriched Artemia fed to adult zebrafish. Disease tolerance of larval zebrafish against Edwardsiella piscicida was demonstrated by 66.34% cumulative per cent survival (CPS) in the P. ruthenica-exposed group, which was higher than the CPS of the control (46.67%) at 72 h post challenge (hpc). Heat-stressed larvae had 55% CPS in the P. ruthenica-immersed group, while the control had 30% CPS at 60 hpc. Immune-stress response gene transcripts (muc5.1, muc5.2, muc5.3, alpi2, alpi3, hsp70, and hsp90a) were induced, while pro-inflammatory genes (tnfα, il1b, and il6) were downregulated in P. ruthenica-immersed larvae compared to the control. This trend was confirmed by low pro-inflammatory and high stress-responsive protein expression levels in P. ruthenica-exposed larvae. Adult zebrafish had higher CPS (27.2%) in the P. ruthenica-fed group than the control (9.52%) upon E. piscicida challenge, suggesting increased disease tolerance. Histological analysis demonstrated modulation of goblet cell density and average villus height in the P. ruthenica-supplemented group. Metagenomics analysis clearly indicated modulation of alpha diversity indices and the relative abundance of Proteobacteria in the P. ruthenica-supplemented zebrafish gut. Furthermore, increased Firmicutes colonisation and reduced Bacteroidetes abundance in the gut were observed upon P. ruthenica supplementation. Additionally, this study confirmed the concentration-dependent increase of colony dispersion and macrophage uptake upon mucin treatment. In summary, P. ruthenica possesses remarkable functional properties as a probiotic that enhances host defence against diseases and thermal stress. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.title | Probiotic effects of Pseudoalteromonas ruthenica: Antibacterial, immune stimulation and modulation of gut microbiota composition | - |
dc.type | Article | - |
dc.citation.endPage | 243 | - |
dc.citation.startPage | 229 | - |
dc.citation.title | Fish and Shellfish Immunology | - |
dc.citation.volume | 131 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.contributor.alternativeName | 권개경 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.identifier.bibliographicCitation | Fish and Shellfish Immunology, v.131, pp.229 - 243 | - |
dc.identifier.doi | 10.1016/j.fsi.2022.09.070 | - |
dc.identifier.scopusid | 2-s2.0-85140036574 | - |
dc.identifier.wosid | 000876610900003 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.subject.keywordAuthor | Pseudoalteromonas ruthenica | - |
dc.subject.keywordAuthor | Probiotics | - |
dc.subject.keywordAuthor | Bioencapsulation | - |
dc.subject.keywordAuthor | Metagenomics | - |
dc.subject.keywordAuthor | Disease tolerance | - |
dc.subject.keywordAuthor | Zebrafish | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |