Proteomic characterization of the sulfur-reducing hyperthermophilic archaeon Thermococcus onnurineus NA1 by 2-DE/MS-MS SCIE SCOPUS

Cited 12 time in WEB OF SCIENCE Cited 11 time in Scopus
Title
Proteomic characterization of the sulfur-reducing hyperthermophilic archaeon Thermococcus onnurineus NA1 by 2-DE/MS-MS
Author(s)
Kwon, Sang Oh; Kang, Sung Gyun; Park, Soon-Ho; Kim, Young Hwan; Choi, Jong-Soon; Lee, Jung-Hyun; Kim, Seung Il
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2009-03
Abstract
Thermococcus onnurineus NA1, a sulfur-reducing hyperthermophilic archaeon, was isolated from a deep-sea hydrothermal vent area in Papua New Guinea. The strain requires elemental sulfur as a terminal electron acceptor for heterotrophic growth on peptides, amino acids and sugars. Recently, genome sequencing of Thermococcus onnurineus NA1 was completed. In this study, 2-DE/MS-MS analysis of the cytosolic proteome was performed to elucidate the metabolic characterization of Thermococcus onnurineus NA1 at the protein level. Among the 1,136 visualized protein spots, 110 proteins were identified. Enzymes related to metabolic pathways of amino acids utilization, glycolysis, pyruvate conversion, ATP synthesis, and protein synthesis were identified as abundant proteins, highlighting the fact that these are major metabolic pathways in Thermococcus onnurineus NA1. Interestingly, multiple spots of phosphoenolpyruvate synthetase and elongation factor Tu were found on 2D gels generated by truncation at the N-terminus, implicating the cellular regulatory mechanism of this key enzyme by protease degradation. In addition to the proteins involved in metabolic systems, we also identified various proteases and stress-related proteins. The proteomic characterization of abundantly induced proteins using 2-DE/MS-MS enables a better understanding of Thermococcus onnurineus NA1 metabolism.
ISSN
1431-0651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4323
DOI
10.1007/s00792-008-0220-4
Bibliographic Citation
EXTREMOPHILES, v.13, no.2, pp.379 - 387, 2009
Publisher
SPRINGER JAPAN KK
Subject
SULFOLOBUS-SOLFATARICUS P2; COMPLETE GENOME SEQUENCE; PYROCOCCUS-HORIKOSHII; AMINOPEPTIDASE; METABOLISM; CLONING
Keywords
Thermococcus onnurineus NA1; Hyperthermophilic archaea; Metabolic pathway analysis; 2-DE/MS-MS
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER JAPAN KK
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