Water hardness alleviates the stress response caused by waterborne zinc in goldfish Carassius auratus SCIE SCOPUS

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Title
Water hardness alleviates the stress response caused by waterborne zinc in goldfish Carassius auratus
Author(s)
Li, Zhongze; Song, Jin Ah; Kim, Min Ju; Choi, Cheol Young
KIOST Author(s)
Song, Jin Ah(송진아)
Alternative Author(s)
송진아
Publication Year
2022-10
Abstract
In this study, the combined effect of waterborne Zn and water hardness on the stress response in the goldfish Carassius auratus was investigated. Goldfish were exposed to Zn concentrations of 0.5, 1.0, and 3.0 mg/L and water hardness of 90, 270, and 450 mg/L CaCO3 for 1, 3, 7, and 14 d. After exposure, it was determined that higher the Zn concentration, the more obvious the stress response. However, the stress response reduced with increasing water hardness. An increase in the Zn concentration caused stress responses in fish according to the increase in the mRNA expressions of corticotropin-releasing hormone and adrenocorticotropic hormone and cortisol level in the hypothalamus–pituitary–interrenal axis. The expression of these factors was the highest on day 7 and decreased on day 14. Furthermore, to evaluate the stress change in the liver tissue, we analyzed alanine aminotransferase, aspartate aminotransferase, and heat shock protein 70 concentrations to determine the damage caused by Zn and the change in water hardness. Immunohistochemistry staining for Na+/K+-ATPase in the gills showed that the gill activity was inhibited by Zn, and an increase in water hardness could improve Na+/K+-ATPase. In conclusion, we found that increasing water hardness is a successful method to reduce the stress response in goldfish caused by Zn. © 2022 Elsevier Inc.
ISSN
0016-6480
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/43113
DOI
10.1016/j.ygcen.2022.114095
Bibliographic Citation
General and Comparative Endocrinology, v.327, 2022
Publisher
Academic Press
Keywords
Goldfish; Hypothalamus–pituitary–interrenal axis; Na+/K+-ATPase; Water hardness; Zinc toxicity
Type
Article
Language
English
Document Type
Article
Publisher
Academic Press
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