OPTIMAL GROWTH CONDITIONS AND ANTIOXIDATIVE ACTIVITIES OF CYLINDROTHECA CLOSTERIUM (BACILLARIOPHYCEAE)1 SCIE SCOPUS

Cited 6 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
OPTIMAL GROWTH CONDITIONS AND ANTIOXIDATIVE ACTIVITIES OF CYLINDROTHECA CLOSTERIUM (BACILLARIOPHYCEAE)1
Author(s)
Affan, Abu; Heo, Soo-Jin; Jeon, You-Jin; Lee, Joon-Baek
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2009-12
Abstract
We isolated the unialgal strain of Cylindotheca closterium (Ehrenb.) Reimann et J. C. Lewin and produced an axenic strain using an antibiotic cocktail of enriched f/2 artificial seawater medium. The optimal growth conditions were estimated under 27 different combinations of temperature, salinity, and nutrients, and mass culture was performed based on the best specific growth conditions. Its antioxidant activities were determined from the extracts of methanol, water, and enzymes (proteases and carbohydrases). The maximum specific growth rate (mu(max)) varied from 0.63 to 0.97 center dot d-1. The maximum cell density was 7.20 x 104 cells center dot mL-1, while the mu(max) was 0.82 center dot d-1 in culture conditions of 20 degrees C, 30 psu (practical salinity unit), and "F" nutrient concentrations on day 10 of the culture period. The scavenging rates for 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical were 72.5% and 69.4% from Viscozyme and methanol extracts, respectively. The enzymatic extracts of C. closterium prepared by the hydrolyses of Amyloglucosidase (AMG) and Viscozyme showed 45.8% and 45.5% nitric-oxide-scavenging rates, slightly lower than the activity of alpha-tocopherol (alpha-tocopherol) but similar to butylated hydroxytoluene (BHT). The extract from methanol and water showed 44.8% and 44.4% scavenging rates, statistically similar with BHT. The metal-chelating activities of the Kojizyme, Alcalase, methanol, Viscozyme, and Neutrase extracts were 67.1, 53.9, 53.2, 52.1, and 50.2 %, respectively, five to six times higher than the commercial antioxidants. The AMG, Viscozyme, and Neutrase extracts showed a remarkable linoleic acid peroxidation inhibition, which was higher than BHT and statistically similar with alpha-tocopherol.
ISSN
0022-3646
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4234
DOI
10.1111/j.1529-8817.2009.00763.x
Bibliographic Citation
JOURNAL OF PHYCOLOGY, v.45, no.6, pp.1405 - 1415, 2009
Publisher
WILEY
Subject
RADICAL SCAVENGING ACTIVITIES; NITRIC-OXIDE; MARINE; SUPEROXIDE; MICROALGA; OXIDATION; EXTRACT; OXYGEN; DIATOM; BACILLARIOPHYCEAE
Keywords
antioxidant activities; benthic diatoms; Cylindrotheca; growth characteristics; mass culture
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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