Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants SCIE SCOPUS

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Title
Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants
Author(s)
Gang, Yehui; Eom, Tae Yang; Marasinghe, Svini Dileepa; Lee, Youngdeuk; Jo, Eunyoung; Oh, Chulhong
KIOST Author(s)
Gang, Ye Hui(강예희)Eom, Tae Yang(엄태양)Marasinghe, Svini Dileepa(Svini)Lee, Young Deuk(이영득)Jo, Eunyoung(조은영)Oh, Chul Hong(오철홍)
Alternative Author(s)
강예희; 엄태양; Svini; 이영득; 조은영; 오철홍
Publication Year
2021-04
Abstract
Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism-derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.
ISSN
1660-3397
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/42233
DOI
10.3390/md19050256
Bibliographic Citation
MARINE DRUGS, v.19, no.5, 2021
Publisher
MDPI
Subject
ANTIOXIDANT ACTIVITY; PLANT-EXTRACTS
Keywords
DPPH assay; antioxidant activity; marine microorganism; cell-free supernatant; salt
Type
Article
Language
English
Document Type
Article
Publisher
MDPI
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