Acetate-assisted carbon monoxide fermentation of Clostridium sp. AWRP SCIE SCOPUS

DC Field Value Language Kwon, Soo Jae - Lee, Joung Min - Lee, Hyun Sook - 2021-12-21T03:30:06Z - 2021-12-21T03:30:06Z - 2021-12-21 - 2022-02 -
dc.identifier.issn 1359-5113 -
dc.identifier.uri -
dc.description.abstract Acetogens are of great interest on conversion of synthesis gas in that they can efficiently utilize CO-containing gases to produce C2 and higher metabolic products. However, high concentration of CO is known to inhibit the reduction of CO2 into formate, thereby diminishing an overall carbon fixation rate. We report that acetate supplementation can be a simple, effective approach to mitigating CO inhibition in Clostridium sp. AWRP (hereinafter called AWRP). The added acetate rapidly reduced into ethanol by the AWRP cells, which coincided with CO oxidation. From stoichiometric calculations, the acetate reduction was observed to allow carbon fixation, indicating that the CO oxidation might reduce the intracellular CO levels below inhibitory levels. Furthermore, AWRP exhibited an 83% higher maximum specific growth rate (μmax) in the acetate-supplemented bottle cultures than in the control. In the bioreactor experiments, the AWRP culture with exogenous acetate displayed 2.9- and 2.3-fold higher titers of ethanol (232 mM) and 2,3-butanediol (23 mM), respectively, as well as μmax (2.6-fold) and maximum cell density (1.9-fold), than without acetate supplementation. These results indicate that this approach can be applied to various fermentative experiments using CO-containing gases to shorten the overall cultivation time. -
dc.description.uri 1 -
dc.language English -
dc.publisher Elsevier Applied Science -
dc.title Acetate-assisted carbon monoxide fermentation of Clostridium sp. AWRP -
dc.type Article -
dc.citation.endPage 54 -
dc.citation.startPage 47 -
dc.citation.title Process Biochemistry -
dc.citation.volume 113 -
dc.contributor.alternativeName 권수재 -
dc.contributor.alternativeName 이종민 -
dc.contributor.alternativeName 이현숙 -
dc.identifier.bibliographicCitation Process Biochemistry, v.113, pp.47 - 54 -
dc.identifier.doi 10.1016/j.procbio.2021.12.015 -
dc.identifier.scopusid 2-s2.0-85121693367 -
dc.identifier.wosid 000736570800006 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.description.isOpenAccess N -
dc.subject.keywordPlus SYNTHESIS GAS SYNGAS -
dc.subject.keywordPlus CO METABOLISM -
dc.subject.keywordPlus ACTIVE-SITE -
dc.subject.keywordPlus SP-NOV -
dc.subject.keywordPlus AUTOETHANOGENUM -
dc.subject.keywordPlus ETHANOL -
dc.subject.keywordPlus LJUNGDAHLII -
dc.subject.keywordPlus REDUCTION -
dc.subject.keywordPlus FORMATE -
dc.subject.keywordPlus ALCOHOLS -
dc.subject.keywordAuthor Acetogen -
dc.subject.keywordAuthor Clostridium -
dc.subject.keywordAuthor CO fermentation -
dc.subject.keywordAuthor Acetate supplementation -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology -
dc.relation.journalWebOfScienceCategory Engineering, Chemical -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology -
dc.relation.journalResearchArea Engineering -
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Marine Resources Research Division > Marine Biotechnology Research Center > 1. Journal Articles
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