A Novel Pseudoalteromonas xiamenensis Marine Isolate as a Potential Probiotic: Anti-Inflammatory and Innate Immune Modulatory Effects against Thermal and Pathogenic Stresses SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
A Novel Pseudoalteromonas xiamenensis Marine Isolate as a Potential Probiotic: Anti-Inflammatory and Innate Immune Modulatory Effects against Thermal and Pathogenic Stresses
Author(s)
Wasana, Withanage Prasadini; Senevirathne, Amal; Nikapitiya, Chamilani; Eom, Tae-Yang; Lee, Young Deuk; Lee, Jong-Soo; Kang, Do Hyung; Oh, Chul Hong; De Zoysa, Mahanama
KIOST Author(s)
Eom, Tae Yang(엄태양)Lee, Young Deuk(이영득)Kang, Do Hyung(강도형)Oh, Chul Hong(오철홍)
Alternative Author(s)
엄태양; 이영득; 강도형; 오철홍
Publication Year
2021-12
Abstract
A marine bacterial strain was isolated from seawater and characterized for it beneficial probiotic effects using zebrafish as a model system. The strain was identified by morphological, physiological, biochemical, and phylogenetic analyses. The strain was most closely related to Pseudoalteromonas xiamenensis Y2, with 99.66% similarity; thus, we named it Pseudoalteromonas xiamenensis S1131. Improvement of host disease tolerance for the P. xiamenensis isolate was adapted in a zebrafish model using Edwardsiella piscicida challenge. The larvae were pre-exposed to P. xiamenensis prior to E. piscicida challenge, resulting in a 73.3% survival rate compared to a 46.6% survival for the control. The treated larvae tolerated elevated temperatures at 38 °C, with 85% survival, compared to 60% survival for the control. Assessment of immunomodulatory responses at the mRNA level demonstrated the suppression of pro-inflammatory markers tnfα and il6, and upregulation of heat shock protein hsp90 and mucin genes. The same effect was corroborated by immunoblot analysis, revealing significant inhibition of Tnfα and an enhanced expression of the Hsp90 protein. The antibacterial activity of P. xiamenensis may be related to mucin overexpression, which can suppress bacterial biofilm formation and enhance macrophage uptake. This phenomenon was evaluated using nonstimulated macrophage RAW264.7 cells. Further studies may be warranted to elucidate a complete profile of the probiotic effects, to expand the potential applications of the present P. xiamenensis isolate.
ISSN
1660-3397
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41855
DOI
10.3390/md19120707
Bibliographic Citation
Marine Drugs, v.19, no.12, 2021
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Keywords
probiotic; Pseudoalteromonas xiamenensis; zebrafish; mucin; Edwardsiella piscicida
Type
Article
Language
English
Document Type
Article
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
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