Effects of paralytic shellfish poisoning toxin-producing dinoflagellate Gymnodinium catenatum on the marine copepod Tigriopus japonicus

Abstract

To understand how the marine copepod Tigriopus japonicus responds to the toxic marine dinoflagellate Gymnodinium catenatum, we assessed acute toxicity and investigated swimming behavior parameters (e.g., swimming speed, swimming path trajectory, and swimming distance) in response to G. catenatum exposure. In addition, the mRNA expression levels of detoxification-related genes (e.g., phase I cytochrome P450 [CYP] and phase II glutathione-S transferase [GST]) were measured in G. catenatum-exposed copepods. No significant change in survival was observed in response to G. catenatum, but swimming speed was significantly decreased (P < 0.05) at a high concentration of G. catenatum (600 cells/mL). Furthermore, the swimming distance was significantly decreased (P < 0.05) compared to that of the control at 600 cells/mL G. catenatum, while no significant change in swimming path trajectory was observed, suggesting that G. catenatum potentially has adverse effects on the swimming behavior of T. japonicus. In addition, the transcriptional regulation of T. japonicus CYPs and -GSTs were significantly upregulated and downregulated (P < 0.05), respectively, in response to G. catenatum. In particular, certain genes (e.g., CYPs [CYP307E1, CYP3041A1, and CYP3024A2] and GSTs [GST-kappa, GST-mu5, and GST-omega]) were significantly induced (P < 0.05) by G. catenatum, suggesting that these genes likely play a critical role in detoxification mechanisms and might be useful as potential molecular biomarkers in response to G. catenatum exposure. Overall, these results elucidate the potential impacts of the dinoflagellate G. catenatum on the swimming behavior and detoxification system of the marine copepod T. japonicus.