Anti-senescence and anti-wrinkle activities of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts
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Title
- Anti-senescence and anti-wrinkle activities of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts
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Author(s)
- Cho, Su-Hyeon; Ko, Eun-Yi; Heo, Soo-Jin; Kim, Seo-Young; Ahn, Juhee; Kim, Kil-Nam
- KIOST Author(s)
- Heo, Soo Jin(허수진)
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Alternative Author(s)
- 허수진
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Publication Year
- 2021-02
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Abstract
- Objective: To investigate the anti-senescence effect of 3-bromo-4,5- dihydroxybenzaldehyde (BDB) from Polysiphonia morrowii Harvey in human dermal fibroblasts (HDF). Methods: HDF were subjected to treatment of BDB and then treated with hydrogen peroxide (H2O2) to induce premature senescence. Senescence-associated beta-galactosidase (SA-beta-gal) activity in HDF was determined using the SA-beta-gal staining method. Intracellular reactive oxygen species (ROS) production was measured using the 2',7'-dichlorodihydrofluorescein diacetate assay. Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1 (GPX1). In addition, intracellular collagen and collagenase contents were analyzed using the respective ELISA kits. Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content. Results: Treatment of HDF with H2O2 increased the activity of SAP-gal, but BDB pre-treatment resulted in the reduction of SA-beta- gal activity. Moreover, BDB significantly reduced H2O2-induced intracellular ROS production. BDB also markedly increased the level of GPX1, which was inhibited by 400 mu M of H2O2. Furthermore, in in vitro study, BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF. Conclusions: Our results demonstrate that BDB shows anti- senescence and anti-wrinkle activities in vitro.
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ISSN
- 2221-1691
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/41357
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DOI
- 10.4103/2221-1691.303606
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Bibliographic Citation
- ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE, v.11, no.2, pp.74 - 80, 2021
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Publisher
- WOLTERS KLUWER MEDKNOW PUBLICATIONS
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Subject
- INDUCED OXIDATIVE STRESS; BETA-GALACTOSIDASE; IN-VITRO; SENESCENCE; CELLS; EXTRACT; MECHANISMS; BIOMARKER; CULTURE; DAMAGE
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Keywords
- Polysiphonia morrowii Harvey; 3-bromo-4,5-dihydroxybenzaldehyde; Oxidative stress; Human dermal fibroblast; Anti-senescence activity; Anti-wrinkle activity
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Type
- Article
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Language
- English
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Document Type
- Article
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