Species identification of raw fish fillet asserted as Pagrus major in the market using the DNA barcode

Title
Species identification of raw fish fillet asserted as Pagrus major in the market using the DNA barcode
Author(s)
정다금; 이윤호
KIOST Author(s)
Lee, Youn Ho(이윤호)
Publication Year
2012-11-30
Abstract
Pagrus major is a commercially important fish species in Korea. It enjoys high consumer preference and a high price compared to other fishes. Since the morphological characters of some distinguished species are undetectable in the processed fish products, consumers might sometimes get deceived of the species and pay more than the reasonable price. In the present study, we identified the species of raw fish fillet asserted as a red seabream (Pagrus major) in the market to examine whether the fillet products were labelled correctly or not in terms of the species. 22 samples of raw fish fillet asserted as Pagrus major were obtained from fish markets and restaurants in October, 2009 and ten samples were collected in March 2012 for further examination. Three specimens from each sample were analyzed for the DNA barcode using the mtDNA COI sequences. About 700bp region of the COI gene was amplified with a pair of oligonucleotide primers, RajaCOIF and RajaCOIRI. The PCR product was purified and sequenced in both directions. 10 out of 22 samples analyzed turned out to be wrongly labelled in term of the species among the samples collected during 2009. The raw fish fillet of asserted as red seabream were identified from not only from P. major but also from Scienops ocellatus, Seriola lalandi, Trachinotus ovatus, Mugil cephalus and Oreochromis sp.. As for 2012 samples, only one sample was labeled as Hssed fish products, consumers might sometimes get deceived of the species and pay more than the reasonable price. In the present study, we identified the species of raw fish fillet asserted as a red seabream (Pagrus major) in the market to examine whether the fillet products were labelled correctly or not in terms of the species. 22 samples of raw fish fillet asserted as Pagrus major were obtained from fish markets and restaurants in October, 2009 and ten samples were collected in March 2012 for further examination. Three specimens from each
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/41036
Bibliographic Citation
[FISH-BOL 2012] Fish Barcode of Life World Conference, pp.93, 2012
Publisher
FISHBOL, 한국어류학회
Type
Conference
Language
English
Publisher
FISHBOL, 한국어류학회
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