DNA바코드를 이용한 다수종이 포함된 네트 시료에서의 종 탐색

Abstract

Evaluation of species occurrence and distribution is a first step toward biodiversity assessment and is a necessary procedure in the studies of biogeography, conservation biology and ecology. Identification of fish eggs becomes more important for detection of spawning periods and grounds in establishment of their proper management and conservation plans. However, the eggs of most species in Korean waters have trouble in identification of their species. The morphological characters of the eggs are similar in many cases and even overlap among the species.In this study, we developed the species-specific probes and primers from the mitochondrial DNA COI sequences so called the DNA barcode which enabled us to identify the eggs of some fish species in the net samples.Using the probes and primers, we verified the presence of some species in multi-species mixed samples via the process of realtime-PCR which was indicated by the result of next generation sequencing of the samples.nt for detection of spawning periods and grounds in establishment of their proper management and conservation plans. However, the eggs of most species in Korean waters have trouble in identification of their species. The morphological characters of the eggs are similar in many cases and even overlap among the species.In this study, we developed the species-specific probes and primers from the mitochondrial DNA COI sequences so called the DNA barcode which enabled us to identify the eggs of some fish species in the net samples.Using the probes and primers, we verified the presence of some species in multi-species mixed samples via the process of realtime-PCR which was indicated by the result of next generation sequencing of the samples.