유전학적 분석을 통한 마카사르해협의 플랑크톤 군집 분석

Title
유전학적 분석을 통한 마카사르해협의 플랑크톤 군집 분석
Alternative Title
Genetic analysis of the plankton community structure in the Makassar Strait
Author(s)
강미혜; Hagi Yulia Sugeha; Arief Rachman; Susetiono; 이윤호
KIOST Author(s)
Lee, Youn Ho(이윤호)
Publication Year
2014-04-25
Abstract
Zooplankton and phytoplankton consisting of the pelagic ecosystem are mostly too small to be identified based on the morphological characters. Molecular characters instead provide an efficient way of species identification, being especially useful for small organisms. In addition, the molecular characters such as the DNA barcode enable us to identify large amount of samples within a short time. An international initiative, Consortium for the Barcode of Life proposed to use a mitochondrial gene, COI as a global standard for the identification of biological species, so called DNA barcode. In the present analysis, we used the DNA barcode to identify the zooplankton samples collected from the EWIN 2013 international survey carried out in the Makassar Strait from June 5 to June 18, 2013. The plankton samples were collected with NORPAC net (mesh size: 0.33mm) and IKMT (mesh size: 0.5mm). The species composition of each sample was analyzed by massive sequencing of the DNA barcode through Next Generation Sequencing (NGS). Approximately 620 zooplankton OTUs were obtained from the samples indicating high diversity of species in the plankton community. Arthropods are the most abundant organisms in the community (41.9% of the OTUs). Other phyla include Cnidaria (8.5%), Mollusca (3.9%), Chordata (3.2%), Chaetognatha (2.7%), and Nemertea, Annelida and Bryozoa contribute less than 1% each. In addition, 38.6% of the OTUs were uniden useful for small organisms. In addition, the molecular characters such as the DNA barcode enable us to identify large amount of samples within a short time. An international initiative, Consortium for the Barcode of Life proposed to use a mitochondrial gene, COI as a global standard for the identification of biological species, so called DNA barcode. In the present analysis, we used the DNA barcode to identify the zooplankton samples collected from the EWIN 2013 international survey carried out in the Makassar Strait from June 5 to June 18, 2013. The plankton samples were collected with NORPAC net (mesh size: 0.33mm) and IKMT (mesh size: 0.5mm). The species composition of each sample was analyzed by massive sequencing of the DNA barcode through Next Generation Sequencing (NGS). Approximately 620 zooplankton OTUs were obtained from the samples indicating high diversity of species in the plankton community. Arthropods are the most abundant organisms in the community (41.9% of the OTUs). Other phyla include Cnidaria (8.5%), Mollusca (3.9%), Chordata (3.2%), Chaetognatha (2.7%), and Nemertea, Annelida and Bryozoa contribute less than 1% each. In addition, 38.6% of the OTUs were uniden
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40955
Bibliographic Citation
IOC/WESTPAC 9th International scientific symposium, pp.13, 2014
Publisher
IOC/WESTPAC
Type
Conference
Language
English
Publisher
IOC/WESTPAC
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse