유전학적 분석을 통한 마카사르해협의 플랑크톤 군집 분석

Abstract

Zooplankton and phytoplankton consisting of the pelagic ecosystem are mostly too small to be identified based on the morphological characters. Molecular characters instead provide an efficient way of species identification, being especially useful for small organisms. In addition, the molecular characters such as the DNA barcode enable us to identify large amount of samples within a short time. An international initiative, Consortium for the Barcode of Life proposed to use a mitochondrial gene, COI as a global standard for the identification of biological species, so called DNA barcode. In the present analysis, we used the DNA barcode to identify the zooplankton samples collected from the EWIN 2013 international survey carried out in the Makassar Strait from June 5 to June 18, 2013. The plankton samples were collected with NORPAC net (mesh size: 0.33mm) and IKMT (mesh size: 0.5mm). The species composition of each sample was analyzed by massive sequencing of the DNA barcode through Next Generation Sequencing (NGS). Approximately 620 zooplankton OTUs were obtained from the samples indicating high diversity of species in the plankton community. Arthropods are the most abundant organisms in the community (41.9% of the OTUs). Other phyla include Cnidaria (8.5%), Mollusca (3.9%), Chordata (3.2%), Chaetognatha (2.7%), and Nemertea, Annelida and Bryozoa contribute less than 1% each. In addition, 38.6% of the OTUs were uniden useful for small organisms. In addition, the molecular characters such as the DNA barcode enable us to identify large amount of samples within a short time. An international initiative, Consortium for the Barcode of Life proposed to use a mitochondrial gene, COI as a global standard for the identification of biological species, so called DNA barcode. In the present analysis, we used the DNA barcode to identify the zooplankton samples collected from the EWIN 2013 international survey carried out in the Makassar Strait from June 5 to June 18, 2013. The plankton samples were collected with NORPAC net (mesh size: 0.33mm) and IKMT (mesh size: 0.5mm). The species composition of each sample was analyzed by massive sequencing of the DNA barcode through Next Generation Sequencing (NGS). Approximately 620 zooplankton OTUs were obtained from the samples indicating high diversity of species in the plankton community. Arthropods are the most abundant organisms in the community (41.9% of the OTUs). Other phyla include Cnidaria (8.5%), Mollusca (3.9%), Chordata (3.2%), Chaetognatha (2.7%), and Nemertea, Annelida and Bryozoa contribute less than 1% each. In addition, 38.6% of the OTUs were uniden