The complete sequence of mitochondrial DNA of the Argentine skate, Dipturus argentinensis was sequenced as being a circular molecule of 16,907 bp including 2 rRNA, 22 tRNA, 13 protein coding genes (PCGs), and an AT-rich control region. The organization of the PCGs is the same as that found in other Rajidae species. The nucleotide of L-strand is composed of 29.7% A, 27.0% C, 28.7% T, and 14.7% G with a bias toward A+T slightly. Twelve of 13 PCGs are initiated by the ATG codon while COX1 starts with GTG. Only ND4 harbors the incomplete termination codon, TA. All tRNA genes have a typical clover-leaf structure of mitochondrial tRNA with the exception of tRNASerAGY which has a reduced DHU arm. This mitogenome will improve the genetic information of the family Rajidae and enhance understanding the phylogeny and the evolution of the genus Dipturus in relation to the other genera of the family Rajidae.organization of the PCGs is the same as that found in other Rajidae species. The nucleotide of L-strand is composed of 29.7% A, 27.0% C, 28.7% T, and 14.7% G with a bias toward A+T slightly. Twelve of 13 PCGs are initiated by the ATG codon while COX1 starts with GTG. Only ND4 harbors the incomplete termination codon, TA. All tRNA genes have a typical clover-leaf structure of mitochondrial tRNA with the exception of tRNASerAGY which has a reduced DHU arm. This mitogenome will improve the genetic information of the family Rajidae and enhance understanding the phylogeny and the evolution of the genus Dipturus in relation to the other genera of the family Rajidae.