Biochemical Characterization of Soluble Acid and Alkaline Invertases from Shoots of Etiolated Pea Seedlings SCIE SCOPUS

Cited 5 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Biochemical Characterization of Soluble Acid and Alkaline Invertases from Shoots of Etiolated Pea Seedlings
Author(s)
Kim, Donggiun; Park, So Yun; Chung, Youngjae; Park, Jongbum; Lee, Sukchan; Lee, Taek-Kyun
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2010-06
Abstract
Soluble invertase was purified from pea (Pisum sativum L.) by sequential procedures entailing ammonium sulfate precipitation, DEAE-Sepharose column, Con-A- and Green 19-Sepharose affinity columns, hydroxyapatite column, ultra-filtration, and Sephacryl 300 gel filtration. The purified soluble acid (SAC) and alkaline (SALK) invertases had a pH optimum of 5.3 and 7.3, respectively. The temperature optimum of two invertases was 37 degrees C. The effects of various concentrations of Tris-HCl, HgCl2, and CuSO4 on the activities of the two purified enzymes were examined. Tris-HCl and HgCl2 did not affect SAC activity, whereas 10 mM Tris-HCl and 0.05 mM HgCl2 inhibited SALK activity by about 50%. SAC and SALK were inhibited by 4.8 mM and 0.6 mM CuSO4 by 50%, respectively. The enzymes display typical hyperbolic saturation kinetics for sucrose hydrolysis. The Kms of SAC and SALK were determined to be 1.8 and 38.6 mM, respectively. The molecular masses of SAC shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting were 22 kDa and 45 kDa. The molecular mass of SALK was 30 kDa. Iso-electric points of the SAC and SALK were estimated to be about pH 7.0 and pH 5.7, respectively.
ISSN
1672-9072
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4087
DOI
10.1111/j.1744-7909.2010.00937.x
Bibliographic Citation
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, v.52, no.6, pp.536 - 548, 2010
Publisher
WILEY-BLACKWELL
Subject
BETA-FRUCTOFURANOSIDASE; STORAGE ROOTS; DAUCUS-CAROTA; POLYACRYLAMIDE GELS; NEUTRAL INVERTASE; SUCROSE SYNTHASE; TOMATO FRUIT; PURIFICATION; LEAVES; VULGARIS
Type
Article
Language
English
Document Type
Article
Publisher
WILEY-BLACKWELL
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