Complete mitochondrial genome of biraphid benthic diatom, Navicula ramosissima (Naviculales, Bacillariophyceae)

Abstract

The complete mitochondrial DNA of biraphid benthic diatom, Navicula ramosissima TA439 was sequenced and characterized. The circular mitogenome contains 67 genes in 48,652 bp (31.1% GC), including 41 protein-coding, 24 tRNA, 2 rRNA genes. 24 protein-coding sequences (CDS, 59%) have start with ATG codon and 17 CDS start with alternatives such as ATA (5), ATT (6), TTA (5), and TTG (1). The GC content of tRNA genes (42.1%) is relatively higher than those of the rRNA (35.2%) and CDS (30.5%). Three genes are consisted of multiple exons and introns, i.e., cox1 (3 exons, 2 introns), rps11 (2 exons, 1 intron), rrl (4 exons, 3 introns). Phylogeny of diatoms based on mitogenome data (34 CDS, 8,530 amino acids combined) supports the monophyly of Naviculales, including N. ramosissima (Naviculaceae), Berkeleya fennica (Berkeleyaceae), Fistulifera solaris (Stauroneidaceae), and Phaeodactylum tricornutum (Phaeodactylaceae). Mitogenome data may useful for phylogenetic study of the diatoms and stramenopiles. protein-coding sequences (CDS, 59%) have start with ATG codon and 17 CDS start with alternatives such as ATA (5), ATT (6), TTA (5), and TTG (1). The GC content of tRNA genes (42.1%) is relatively higher than those of the rRNA (35.2%) and CDS (30.5%). Three genes are consisted of multiple exons and introns, i.e., cox1 (3 exons, 2 introns), rps11 (2 exons, 1 intron), rrl (4 exons, 3 introns). Phylogeny of diatoms based on mitogenome data (34 CDS, 8,530 amino acids combined) supports the monophyly of Naviculales, including N. ramosissima (Naviculaceae), Berkeleya fennica (Berkeleyaceae), Fistulifera solaris (Stauroneidaceae), and Phaeodactylum tricornutum (Phaeodactylaceae). Mitogenome data may useful for phylogenetic study of the diatoms and stramenopiles.