Long-term outdoor cultivation by perfusing spent medium for biodiesel production from Chlorella minutissima SCIE SCOPUS

Cited 19 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Long-term outdoor cultivation by perfusing spent medium for biodiesel production from Chlorella minutissima
Author(s)
Oh, Sung Ho; Kwon, Min Chul; Choi, Woon Yong; Seo, Yong Chang; Kim, Ga Bin; Kang, Do Hyung; Lee, Shin Young; Lee, Hyeon Yong
KIOST Author(s)
Kang, Do Hyung(강도형)
Publication Year
2010-08
Abstract
A unique perfusion process was developed to maintain high concentrations of marine alga. Chlorella minutissima. This method is based on recycling cells by continuous feeding with warm spent sea water from nuclear power plants, which has very similar properties as sea water. A temperature of at least 30 degrees C in a 200 L photo-bioreactor was maintained in this system by perfusion of the thermal plume for 80 days in the coldest season. The maximum cell concentration and total lipid content was 8.3 g-dry wt./L and 23.2 %, w/w, respectively, under mixotrophic conditions. Lipid production was found to be due to a partially or non-growth related process, which implies that large amounts of biomass are needed for a high accumulation of lipids within the cells. At perfusion rates greater than 1.5 L/h, the temperature of the medium inside the reactor was around 30 degrees C, which was optimal for cell growth. For this system, a perfusion rate of 2.8 L/h was determined to be optimal for maintaining rapid cell growth and lipid production during outdoor cultivation. It was absolutely necessary to maintain the appropriate perfusion rate so that the medium temperature was optimal for cell growth. In addition, the lipids produced using this process were shown to be feasible for biodiesel production since the lipid composition of C. minutissima grown under these conditions consisted of 17 % (w/w) of C-16 and 47% (w/w) of C-18. The combined results of this study clearly demonstrated that the discharged energy of the thermal plume could be reused to cultivate marine alga by maintaining a relatively constant temperature in an outdoor photo-bioreactor without the need for supplying any extra energy, which could allow for cheap production of biodiesel from waste energy. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
ISSN
1389-1723
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4056
DOI
10.1016/j.jbiosc.2010.02.009
Bibliographic Citation
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.110, no.2, pp.194 - 200, 2010
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
Subject
PROTOTHECOIDES; MICROALGAE; GROWTH; PHOTOBIOREACTOR; CULTURE; ACID
Keywords
200L photo-bioreactor; Thermal plume; Outdoor large cultivation; Mixotrophic growth; Chlorella minutissima
Type
Article
Language
English
Document Type
Article
Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
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