Disk Abalone, Haliotis discus discus, CuZn-Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254 SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | De Zoysa, Mahanama | - |
dc.contributor.author | Ekanayake, Prashani Mudika | - |
dc.contributor.author | Kang, Hyun-Sil | - |
dc.contributor.author | Lee, Jehee | - |
dc.contributor.author | Jee, Youngheun | - |
dc.contributor.author | Lee, Youn-Ho | - |
dc.contributor.author | Kim, Sang-Jin | - |
dc.date.accessioned | 2021-03-17T08:27:55Z | - |
dc.date.accessioned | 2021-03-17T08:27:55Z | - |
dc.date.available | 2021-03-17T08:27:55Z | - |
dc.date.available | 2021-03-17T08:27:55Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.issn | 0893-8849 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/40461 | - |
dc.description.abstract | CuZn-superoxide dismutase (CuZn-SOD) is a key antioxidant enzyme playing a first line protective role against reactive oxygen species (ROS) by converting superoxide (O-2(.)-) into H2O2. The CuZn-SOD gene was isolated from a whole abalone cDNA library and denoted as aCuZn-SOD. The full-length cDNA of aCuZn-SOD was 1021 bp, which contained 465-bp open reading frame (ORF) coding 154 amino acids. It contained highly conserved CuZn-SOD signature motif 1 (45GFHVHQFGDNT55) and motif 2 (139GNAGGRQACGVI150). Also, amino acid residues identified as Cu (His(47), His(49), His(64), and His(121)) and Zn (His(64), His(72), His(81), and ASP(84)) metal-binding sites were completely conserved in the aCuZn-SOD. The reverse transcription polymerase chain reaction (RT-PCR) results showed that aCuZn-SOD mRNA was expressed constitutively in gill, mantle, gonad, abductor muscle, digestive tract, and hemocytes in a tissue-specific manner. The aCuZn-SOD mRNA was significantly up-regulated (P < 0.05) in gill and digestive tract tissues after Aroclor 1254 induction compared to untreated and methanol-injected abalone groups, suggesting that abalone has a potential use in assessing the impact of marine pollutants with the application of gene expression concept. In addition, purified recombinant aCuZn-SOD fusion protein was shown to reduce O-2(.)- radical generated by xanthine oxidase assay, showing CuZn-SOD is a functionally active antioxidant enzyme in disk abalone. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | MYTILUS-GALLOPROVINCIALIS | - |
dc.subject | ANTIOXIDANT ENZYMES | - |
dc.subject | BIOMARKERS | - |
dc.subject | EXPRESSION | - |
dc.subject | FISH | - |
dc.subject | CONTAMINATION | - |
dc.subject | POLLUTION | - |
dc.subject | CLONING | - |
dc.subject | PURIFICATION | - |
dc.title | Disk Abalone, Haliotis discus discus, CuZn-Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254 | - |
dc.type | Article | - |
dc.citation.endPage | 658 | - |
dc.citation.startPage | 643 | - |
dc.citation.title | JOURNAL OF THE WORLD AQUACULTURE SOCIETY | - |
dc.citation.volume | 40 | - |
dc.citation.number | 5 | - |
dc.contributor.alternativeName | 이윤호 | - |
dc.contributor.alternativeName | 김상진 | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE WORLD AQUACULTURE SOCIETY, v.40, no.5, pp.643 - 658 | - |
dc.identifier.doi | 10.1111/j.1749-7345.2009.00284.x | - |
dc.identifier.scopusid | 2-s2.0-70350408729 | - |
dc.identifier.wosid | 000270428600006 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | MYTILUS-GALLOPROVINCIALIS | - |
dc.subject.keywordPlus | ANTIOXIDANT ENZYMES | - |
dc.subject.keywordPlus | BIOMARKERS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FISH | - |
dc.subject.keywordPlus | CONTAMINATION | - |
dc.subject.keywordPlus | POLLUTION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |