Disk Abalone, Haliotis discus discus, CuZn-Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254 SCIE SCOPUS

Cited 3 time in WEB OF SCIENCE Cited 3 time in Scopus
Title
Disk Abalone, Haliotis discus discus, CuZn-Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254
Author(s)
De Zoysa, Mahanama; Ekanayake, Prashani Mudika; Kang, Hyun-Sil; Lee, Jehee; Jee, Youngheun; Lee, Youn-Ho; Kim, Sang-Jin
KIOST Author(s)
Lee, Youn Ho(이윤호)
Publication Year
2009-10
Abstract
CuZn-superoxide dismutase (CuZn-SOD) is a key antioxidant enzyme playing a first line protective role against reactive oxygen species (ROS) by converting superoxide (O-2(.)-) into H2O2. The CuZn-SOD gene was isolated from a whole abalone cDNA library and denoted as aCuZn-SOD. The full-length cDNA of aCuZn-SOD was 1021 bp, which contained 465-bp open reading frame (ORF) coding 154 amino acids. It contained highly conserved CuZn-SOD signature motif 1 (45GFHVHQFGDNT55) and motif 2 (139GNAGGRQACGVI150). Also, amino acid residues identified as Cu (His(47), His(49), His(64), and His(121)) and Zn (His(64), His(72), His(81), and ASP(84)) metal-binding sites were completely conserved in the aCuZn-SOD. The reverse transcription polymerase chain reaction (RT-PCR) results showed that aCuZn-SOD mRNA was expressed constitutively in gill, mantle, gonad, abductor muscle, digestive tract, and hemocytes in a tissue-specific manner. The aCuZn-SOD mRNA was significantly up-regulated (P < 0.05) in gill and digestive tract tissues after Aroclor 1254 induction compared to untreated and methanol-injected abalone groups, suggesting that abalone has a potential use in assessing the impact of marine pollutants with the application of gene expression concept. In addition, purified recombinant aCuZn-SOD fusion protein was shown to reduce O-2(.)- radical generated by xanthine oxidase assay, showing CuZn-SOD is a functionally active antioxidant enzyme in disk abalone.
ISSN
0893-8849
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40461
DOI
10.1111/j.1749-7345.2009.00284.x
Bibliographic Citation
JOURNAL OF THE WORLD AQUACULTURE SOCIETY, v.40, no.5, pp.643 - 658, 2009
Publisher
WILEY
Subject
OXIDATIVE STRESS; MYTILUS-GALLOPROVINCIALIS; ANTIOXIDANT ENZYMES; BIOMARKERS; EXPRESSION; FISH; CONTAMINATION; POLLUTION; CLONING; PURIFICATION
Type
Article
Language
English
Document Type
Article
Publisher
WILEY
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