Characterization of beta-1,4-endoglucanase as a polysaccharide-degrading digestive enzyme from disk abalone, Haliotis discus discus SCIE SCOPUS

DC Field Value Language
dc.contributor.author Nikapitiya, Chamilani -
dc.contributor.author Oh, Chulhong -
dc.contributor.author De Zoysa, Mahanama -
dc.contributor.author Whang, Ilson -
dc.contributor.author Kang, Do-Hyung -
dc.contributor.author Lee, Sun-Ryung -
dc.contributor.author Kim, Se-Jae -
dc.contributor.author Lee, Jehee -
dc.date.accessioned 2020-04-20T08:25:33Z -
dc.date.available 2020-04-20T08:25:33Z -
dc.date.created 2020-01-28 -
dc.date.issued 2010-10 -
dc.identifier.issn 0967-6120 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/4030 -
dc.description.abstract Gene coding for a putative endoglucanase was isolated and characterized from a disk abalone Haliotis discus discus cDNA library and was named as hdEndgI. The full length (1,908 bp) abalone cDNA contained a 1,824 bp open reading frame (ORF), encoding 608 amino acids (aa). The predicted molecular mass and isoelectric point were 65 kDa and 4.5, respectively. The C-terminal region showed conserved catalytic site regions, with the catalytic site residues responsible for the endoglucanase activity. RT-PCR results revealed greater hdEndgI mRNA expression in the hepatopancreas than in the digestive tract, suggesting the key site at which the enzyme is produced. hdEndgI mRNA expression decreased from baseline to the 8th week of starvation in the hepatopancreas; and once re-feeding was initiated, mRNA transcription increased to normal levels within 14 days. This suggests that gene transcription is not totally disturbed due to starvation-induced stress, showing the potential of hdEndgI as a molecular tool for identification of food availability. It further suggests that abalone can survive for 8 weeks without food. The purified hdEndgI recombinant fusion protein was shown to have in vitro activity against carboxymethylcellulose. The enzyme exhibits a broad range of pH stability, the optimal temperature and pH being 40A degrees C and 4.5, respectively. -
dc.description.uri 1 -
dc.language English -
dc.publisher SPRINGER -
dc.subject THERMOCELLUM ENDOGLUCANASE CELD -
dc.subject CRAYFISH CHERAX-QUADRICARINATUS -
dc.subject PARASITIC CYST NEMATODES -
dc.subject CELLULASE GENE -
dc.subject CDNA CLONING -
dc.subject SEQUENCE ALIGNMENT -
dc.subject LONGICORN BEETLE -
dc.subject REDCLAW CRAYFISH -
dc.subject MYTILUS-EDULIS -
dc.subject BLUE MUSSEL -
dc.title Characterization of beta-1,4-endoglucanase as a polysaccharide-degrading digestive enzyme from disk abalone, Haliotis discus discus -
dc.type Article -
dc.citation.endPage 1078 -
dc.citation.startPage 1061 -
dc.citation.title AQUACULTURE INTERNATIONAL -
dc.citation.volume 18 -
dc.citation.number 6 -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.identifier.bibliographicCitation AQUACULTURE INTERNATIONAL, v.18, no.6, pp.1061 - 1078 -
dc.identifier.doi 10.1007/s10499-010-9324-9 -
dc.identifier.scopusid 2-s2.0-77956323450 -
dc.identifier.wosid 000282316700008 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus THERMOCELLUM ENDOGLUCANASE CELD -
dc.subject.keywordPlus CRAYFISH CHERAX-QUADRICARINATUS -
dc.subject.keywordPlus PARASITIC CYST NEMATODES -
dc.subject.keywordPlus CELLULASE GENE -
dc.subject.keywordPlus CDNA CLONING -
dc.subject.keywordPlus SEQUENCE ALIGNMENT -
dc.subject.keywordPlus LONGICORN BEETLE -
dc.subject.keywordPlus REDCLAW CRAYFISH -
dc.subject.keywordPlus MYTILUS-EDULIS -
dc.subject.keywordPlus BLUE MUSSEL -
dc.subject.keywordAuthor Abalone -
dc.subject.keywordAuthor Beta-1,4-endoglucanase -
dc.subject.keywordAuthor Cellulase -
dc.subject.keywordAuthor mRNA expression -
dc.subject.keywordAuthor Starvation -
dc.relation.journalWebOfScienceCategory Fisheries -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Fisheries -
Appears in Collections:
Jeju Research Institute > Tropical & Subtropical Research Center > 1. Journal Articles
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