MOLECULAR CLONING, CHARACTERIZATION AND ENZYMATIC PROPERTIES OF A NOVEL BETA-AGARASE FROM A MARINE ISOLATE PSUDOALTEROMONAS SP. AG52
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Title
- MOLECULAR CLONING, CHARACTERIZATION AND ENZYMATIC PROPERTIES OF A NOVEL BETA-AGARASE FROM A MARINE ISOLATE PSUDOALTEROMONAS SP. AG52
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Author(s)
- Oh, Chulhong; Nikapitiya, Chamilani; Lee, Youngdeuk; Whang, Ilson; Kang, Do-Hyung; Heo, Soo-Jin; Choi, Young-Ung; Lee, Jehee
- KIOST Author(s)
- Oh, Chul Hong(오철홍); Kang, Do Hyung(강도형); Heo, Soo Jin(허수진); Choi, Young Ung(최영웅)
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Alternative Author(s)
- 오철홍; 강도형; 허수진; 최영웅
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Publication Year
- 2010-10
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Abstract
- An agar-degrading Pseudoalteromonas sp. AG52 bacterial strain was identified from the red seaweed Gelidium amansii collected from Jeju Island, Korea. A beta-agarase gene which has 96.8% nucleotide identity to Aeromonas beta-agarase was cloned from this strain, and was designated as agaA. The coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (GHF)-16. The predicted molecular mass of the mature protein was 32 kDa. The recombinant beta-agarase (rAgaA) was overexpressed in Escherichia coli and purified as a fusion protein. The optimal temperature and pH for activity were 55 degrees C and 5.5, respectively. The enzyme had a specific activity of 105.1 and 79.5 unit/mg toward agar and agarose, respectively. The pattern of agar hydrolysis demonstrated that the enzyme is an endo-type beta-agarase, producing neoagarohexaose and neoagarotetraose as the final main products. Since, Pseudoalteromonas sp. AG52 encodes an agaA gene, which has greater identity to Aeromonas beta-agarase, the enzyme could be considered as novel, with its unique bio chemical characteristics. Altogether, the purified rAgaA has potential for use in industrial applications such as development of cosmetics and pharmaceuticals.
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ISSN
- 1517-8382
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/4028
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DOI
- 10.1590/S1517-83822010000400006
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Bibliographic Citation
- BRAZILIAN JOURNAL OF MICROBIOLOGY, v.41, no.4, pp.876 - 889, 2010
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Publisher
- SOC BRASILEIRA MICROBIOLOGIA
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Subject
- DEEP-SEA MICROBULBIFER; AMINO-ACID-SEQUENCES; EXTRACELLULAR-AGARASE; PSEUDOMONAS-ATLANTICA; SUBTILISIN CARLSBERG; GLYCOSIDE HYDROLASES; BACILLUS-MOJAVENSIS; RECOMBINANT ENZYME; KAPPA-CARRAGEENASE; STRAIN JT0107
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Keywords
- Agar; Aeromonas sp.; beta-agarase; Pseudoalteromonas sp.; GHF-16; Neoagarooligosaccharides
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Type
- Article
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Language
- English
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Document Type
- Article
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