MOLECULAR CLONING, CHARACTERIZATION AND ENZYMATIC PROPERTIES OF A NOVEL BETA-AGARASE FROM A MARINE ISOLATE PSUDOALTEROMONAS SP. AG52 SCIE SCOPUS

Cited 15 time in WEB OF SCIENCE Cited 16 time in Scopus
Title
MOLECULAR CLONING, CHARACTERIZATION AND ENZYMATIC PROPERTIES OF A NOVEL BETA-AGARASE FROM A MARINE ISOLATE PSUDOALTEROMONAS SP. AG52
Author(s)
Oh, Chulhong; Nikapitiya, Chamilani; Lee, Youngdeuk; Whang, Ilson; Kang, Do-Hyung; Heo, Soo-Jin; Choi, Young-Ung; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)Heo, Soo Jin(허수진)
Publication Year
2010-10
Abstract
An agar-degrading Pseudoalteromonas sp. AG52 bacterial strain was identified from the red seaweed Gelidium amansii collected from Jeju Island, Korea. A beta-agarase gene which has 96.8% nucleotide identity to Aeromonas beta-agarase was cloned from this strain, and was designated as agaA. The coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (GHF)-16. The predicted molecular mass of the mature protein was 32 kDa. The recombinant beta-agarase (rAgaA) was overexpressed in Escherichia coli and purified as a fusion protein. The optimal temperature and pH for activity were 55 degrees C and 5.5, respectively. The enzyme had a specific activity of 105.1 and 79.5 unit/mg toward agar and agarose, respectively. The pattern of agar hydrolysis demonstrated that the enzyme is an endo-type beta-agarase, producing neoagarohexaose and neoagarotetraose as the final main products. Since, Pseudoalteromonas sp. AG52 encodes an agaA gene, which has greater identity to Aeromonas beta-agarase, the enzyme could be considered as novel, with its unique bio chemical characteristics. Altogether, the purified rAgaA has potential for use in industrial applications such as development of cosmetics and pharmaceuticals.
ISSN
1517-8382
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/4028
DOI
10.1590/S1517-83822010000400006
Bibliographic Citation
BRAZILIAN JOURNAL OF MICROBIOLOGY, v.41, no.4, pp.876 - 889, 2010
Publisher
SOC BRASILEIRA MICROBIOLOGIA
Subject
DEEP-SEA MICROBULBIFER; AMINO-ACID-SEQUENCES; EXTRACELLULAR-AGARASE; PSEUDOMONAS-ATLANTICA; SUBTILISIN CARLSBERG; GLYCOSIDE HYDROLASES; BACILLUS-MOJAVENSIS; RECOMBINANT ENZYME; KAPPA-CARRAGEENASE; STRAIN JT0107
Keywords
Agar; Aeromonas sp.; beta-agarase; Pseudoalteromonas sp.; GHF-16; Neoagarooligosaccharides
Type
Article
Language
English
Document Type
Article
Publisher
SOC BRASILEIRA MICROBIOLOGIA
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