Quantification of triiodothyronine and thyroxine in rat serum using liquid chromatography tandem mass spectrometry SCIE SCOPUS

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Title
Quantification of triiodothyronine and thyroxine in rat serum using liquid chromatography tandem mass spectrometry
Author(s)
Ko, A-Yeong; Yang, Jun-Young; Kim, Donghwi; Eom, Han Young; Lee, Jong-Hwa
KIOST Author(s)
Kim, Dong Hwi(김동휘)
Publication Year
2021-02-20
Abstract
Thyroid hormones act on almost every tissue in the body to promote catabolism in cells and are important for regulating many biological processes. Accurate quantification of endogenous thyroid hormones has become essential for clinical and non-clinical applications in the development of new drugs according to the OECD Guideline (2018). However, there are difficulties in quantitative analysis of thyroid hormones because no analyte-free biological matrices are available for analysis of endogenous substances. In this study, surrogate matrix and surrogate analyte methods were compared and validated to quantify endogenous triiodothyronine (T-3) and thyroxine (T-4) in rat serum using LC-MS/MS. Separation of analytes was performed using an Xbridge (TM) C18 (2.1 x 50 mm, 2.5 mu m) column. In the surrogate matrix, 3,3'5-triiodo- L-thyronine-C-13(6) (cT(3)) and L-thyroxine-C-13(6) (cT(4)) were used as the internal standard (IS), and in the surrogate analyte, L-3,3'-diiodothyronine-C-13(6)(cT(2)) was used as the IS. The mobile phases consisted of 0.1 % acetic acid in purified water (A) and 0.1 % acetic acid in acetonitrile (B). Both analytical methods were suitable for selectivity, matrix effect, carryover, lower limit of quantification, linearity, accuracy, precision, recovery, stability and parallelism. The surrogate matrix method was more accurate than using the surrogate analyte method, including evaluation of parallelism at low concentrations. Additionally, the surrogate matrix is cost-effective for T-3 and T-4 analysis in biological samples because it consists only of deionized water. However, surrogate analytes difficult to evaluate parallelism by obtaining response factors for mass spectrometric signal differences between the actual and surrogate analytes. Therefore, the results of this study indicate that it is more cost-effective to use the surrogate matrix method for endogenous thyroid hormone, T-3 and T-4, analysis in biological samples. (C) 2020 Elsevier B.V. All rights reserved.
ISSN
0731-7085
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/40163
DOI
10.1016/j.jpba.2020.113840
Bibliographic Citation
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.195, 2021
Publisher
ELSEVIER
Subject
BINDING
Keywords
Triiodothyronine; Thyroxine; LC-MS/MS; Quantification; Surrogate
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER
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