Microarray analysis of gene expression in disk abalone Haliotis discus discus after bacterial challenge SCIE SCOPUS

Cited 17 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Microarray analysis of gene expression in disk abalone Haliotis discus discus after bacterial challenge
Author(s)
De Zoysa, Mahanama; Nikapitiya, Chamilani; Oh, Chulhong; Lee, Youngdeuk; Whang, Ilson; Lee, Jae-Seong; Choi, Cheol Young; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)
Publication Year
2011-02
Abstract
In this study, we investigated the gene expression profiling of disk abalone, Haliotis discus discus challenged by a mixture of three pathogenic bacteria Vibrio alginolyticus, Vibrio parahemolyticus, and Listeria monocytogenes using a cDNA microarray. Upon bacteria challenge, 68 (1.6%) and 112 (2.7%) gene transcripts changed their expression levels >= 2 or <= 2 -fold in gills and digestive tract, respectively. There were 46 tissue-specific transcripts that up-regulated specifically in the digestive tract. In contrast, only 13 transcripts showed gill-specific up-regulation. Quantitative real-time PCR was performed to verify microarray data and results revealed that candidate genes namely Kruppell-like factor (KLF), lachesin, muscle lim protein, thioredoxin-2 (TRx-2), nuclear factor interleukin 3 (NFIL-3) and abalone protein 38 were up-regulated. Also, our results further indicated that bacteria challenge may activate the transcription factors or their activators (Kruppell-like factor, inhibitor of NF-kappa B or Ik-B), inflammatory cytokines (IL-3 regulated protein, allograft inflammatory factor), other cytokines (IFN-44-like protein, SOCS-2), antioxidant enzymes (glutathione-S-transferase, thioredoxin-2 and thioredoxin peroxidase), and apoptosis-related proteins (TNF-alpha, archeron) in abalone. The identification of immune and stress response genes and their expression profiles in this microarray will permit detailed investigation of the stress and immune responses of abalone genes. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3918
DOI
10.1016/j.fsi.2010.12.021
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.30, no.2, pp.661 - 673, 2011
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
TUMOR-NECROSIS-FACTOR; DIVERSICOLOR-SUPERTEXTA; CLONING; CDNA; IDENTIFICATION; DISEASE; VIRUS; INTERFERON; MECHANISMS; MORTALITY
Keywords
Abalone; Bacteria; cDNA microarray; Innate immunity; Invertebrate; Mollusk
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Related Researcher
Research Interests

Recombinant protein,Marine microorganisms,Functional proteins,재조합단백질,해양미생물,기능성단백질

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