Molecular characterization and expression analysis of Cathepsin B and L cysteine proteases from rock bream (Oplegnathus fasciatus) SCIE SCOPUS
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Whang, Ilson | - |
dc.contributor.author | De Zoysa, Mahanama | - |
dc.contributor.author | Nikapitiya, Chamilani | - |
dc.contributor.author | Lee, Youngdeuk | - |
dc.contributor.author | Kim, Yucheol | - |
dc.contributor.author | Lee, Sukkyoung | - |
dc.contributor.author | Oh, Chulhong | - |
dc.contributor.author | Jung, Sung-Ju | - |
dc.contributor.author | Oh, Myung-Joo | - |
dc.contributor.author | Choi, Cheol Young | - |
dc.contributor.author | Yeo, Sang-Yeob | - |
dc.contributor.author | Kim, Bong-Seok | - |
dc.contributor.author | Kim, Se-Jae | - |
dc.contributor.author | Lee, Jehee | - |
dc.date.accessioned | 2020-04-20T07:55:04Z | - |
dc.date.available | 2020-04-20T07:55:04Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3911 | - |
dc.description.abstract | Cathepsins are lysosomal cysteine proteases of the papain family that play an important role in intracellular protein degradation and turn over within the lysosomal system. In the present study, full-length sequences of cathepsin B (RbCathepsin B) and L (RbCathepsin L) were identified after transcriptome sequencing of rock bream Oplegnathus fasciatus mixed tissue cDNA. Cathepsin B was composed of 330 amino acid residues with 36 kDa predicted molecular mass. RbCathepsin L contained 336 amino acid residues encoding for a 38 kDa predicted molecular mass protein. The sequencing analysis results showed that both cathepsin B and L contain the characteristic papain family cysteine protease signature and active sites for the eukaryotic thiol proteases of cysteine, asparagine and histidine. In addition, RbCathepsin L contained EF hand Ca2+ binding and cathepsin propeptide inhibitor domains. The rock bream cathepsin B and L showed the highest amino acid identity of 90 and 95% to Lutjanus argentimaculatus cathepsin B and Lates calcarifer cathepsin L, respectively. By phylogenetic analysis, cathepsin B and L exhibited a high degree of evolutionary relationship to respective cathepsin family members of the papain superfamily. Quantitative real-time RT-PCR analysis results confirmed that the expression of cathepsin B and L genes was constitutive in all examined tissues isolated from un-induced rock bream. Moreover, activation of RbCathepsin B and L mRNA was observed in both lipopolysaccharide (LPS) and Edwardsiella tarda challenged liver and blood cells, indicating a role of immune response in rock bream. (C) 2010 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.subject | TURBOT SCOPHTHALMUS-MAXIMUS | - |
dc.subject | EDWARDSIELLA-TARDA GHOSTS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | PARALICHTHYS-OLIVACEUS | - |
dc.subject | SEQUENCE ALIGNMENT | - |
dc.subject | IDENTIFICATION | - |
dc.subject | PROTEIN | - |
dc.subject | ENZYME | - |
dc.subject | VIRUS | - |
dc.subject | CELLS | - |
dc.title | Molecular characterization and expression analysis of Cathepsin B and L cysteine proteases from rock bream (Oplegnathus fasciatus) | - |
dc.type | Article | - |
dc.citation.endPage | 772 | - |
dc.citation.startPage | 763 | - |
dc.citation.title | FISH & SHELLFISH IMMUNOLOGY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 오철홍 | - |
dc.identifier.bibliographicCitation | FISH & SHELLFISH IMMUNOLOGY, v.30, no.3, pp.763 - 772 | - |
dc.identifier.doi | 10.1016/j.fsi.2010.12.022 | - |
dc.identifier.scopusid | 2-s2.0-79952189883 | - |
dc.identifier.wosid | 000288888500002 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TURBOT SCOPHTHALMUS-MAXIMUS | - |
dc.subject.keywordPlus | EDWARDSIELLA-TARDA GHOSTS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PARALICHTHYS-OLIVACEUS | - |
dc.subject.keywordPlus | SEQUENCE ALIGNMENT | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Cathepsin | - |
dc.subject.keywordAuthor | Lysosomal cysteine proteases | - |
dc.subject.keywordAuthor | Papain superfamily | - |
dc.subject.keywordAuthor | Rock bream | - |
dc.subject.keywordAuthor | Oplegnathus fasciatus | - |
dc.relation.journalWebOfScienceCategory | Fisheries | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Marine & Freshwater Biology | - |
dc.relation.journalWebOfScienceCategory | Veterinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Fisheries | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Marine & Freshwater Biology | - |
dc.relation.journalResearchArea | Veterinary Sciences | - |