Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Arochlor 1260-exposed Marine Medaka SCOPUS

Title
Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Arochlor 1260-exposed Marine Medaka
Alternative Title
Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Arochlor 1260-exposed Marine Medaka
Author(s)
원효경; 우선옥; 이애경; 이건섭; 이택견; 염승식
KIOST Author(s)
Lee, Na Yun(이나윤)Lee, Taek Kyun(이택견)Yum, Seungshic(염승식)
Publication Year
2012
Abstract
Differential gene expression profiling was performed by heterologous hybridization using a medaka cDNA microarray on the hepatic tissue of the marine medaka (Oryzias javanicus) after exposure to Arochlor 1260, a polychlorinated biphenyl (PCB), which is classified as a persistent organic pollutant. Twenty-eight differentially expressed candidate genes were identified; one was induced and 27 were repressed (P < 0.01). The genes were assembled into 10 groups based mainly on the Eukaryotic Othologous Groups classification. These isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for identifying environmental stressors. The results obtained in this study will allow future studies to assess the molecular mechanisms of Arochlor 1260 toxicity and to develop a systems biology approach to PCB stress biology. (PCB), which is classified as a persistent organic pollutant. Twenty-eight differentially expressed candidate genes were identified; one was induced and 27 were repressed (P < 0.01). The genes were assembled into 10 groups based mainly on the Eukaryotic Othologous Groups classification. These isolated gene candidates were differentially expressed and therefore have great potential as molecular biomarkers for identifying environmental stressors. The results obtained in this study will allow future studies to assess the molecular mechanisms of Arochlor 1260 toxicity and to develop a systems biology approach to PCB stress biology.
ISSN
2005-9752
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38901
Bibliographic Citation
Toxicology and Environmental Health Sciences, v.4, no.2, pp.132 - 138, 2012
Publisher
Korean Society of Environmental Risk Assessment and Health Science
Keywords
Arochlor 1260; Differential gene expression profile; Heterologous hybridization; Marine medaka; Polychlorinated biphenyl (PCB)
Type
Article
Language
English
Publisher
Korean Society of Environmental Risk Assessment and Health Science
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