Antihypertensive effect of surimi prepared from olive flounder (Paralichthys olivaceus) by angiotensin-I converting enzyme (ACE) inhibitory activity and characterization of ACE inhibitory peptides SCIE SCOPUS

Cited 17 time in WEB OF SCIENCE Cited 21 time in Scopus
Title
Antihypertensive effect of surimi prepared from olive flounder (Paralichthys olivaceus) by angiotensin-I converting enzyme (ACE) inhibitory activity and characterization of ACE inhibitory peptides
Author(s)
Oh, Jae-Young; Kim, Eun-A; Lee, Hyogeun; Kim, Hyun-Soo; Lee, Jung-Suck; Jeon, You-Jin
KIOST Author(s)
Kim, Eun A(김은아)
Alternative Author(s)
김은아
Publication Year
2019-05
Abstract
In this study, we investigated the physicochemical characteristics of flounder surimi (FS) and surimi gel (FSG). We analyzed the proximate composition, heavy metal content, and amino acid composition of FS and the gel strength and whiteness of FSG. In spontaneously hypertensive rats, the oral administration of FS significantly decreased systolic blood pressure. We further evaluated the antihypertensive activity of an FS digest (FSD) using an in vitro gastric model. FSD had significant ACE inhibitory activity and increased nitric oxide (NO) levels in human umbilical vein endothelial cells. We purified and identified three peptides from FSD by gel filtration chromatography and quadrupole time of flight electrospray ionization mass spectrometry. We predicted the tertiary structure of ACE for docking simulation. The bioactive peptides from FS showed successful binding to ACE (PDB ID: 1086) with negative binding energy and negative CDOCKER interaction energy in an in silica docking analysis. These results showed that FSD functions as an antihypertensive agent and FS could be a beneficial ingredient in the nutraceutical, pharmaceutical, and functional food industries.
ISSN
1359-5113
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38862
DOI
10.1016/j.procbio.2019.01.016
Bibliographic Citation
PROCESS BIOCHEMISTRY, v.80, pp.164 - 170, 2019
Publisher
ELSEVIER SCI LTD
Keywords
Antihypertensive; Olive flounder; Surimi; Angiotensin-I-converting enzyme; Peptide
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse