Heterosigma akashiwo를 모니터하기 위한 뉴클레아제 보호 분석이 통합된 샌드위치 혼성(NPA-SH)의 개발 KCI

Title
Heterosigma akashiwo를 모니터하기 위한 뉴클레아제 보호 분석이 통합된 샌드위치 혼성(NPA-SH)의 개발
Alternative Title
Development of a Nuclease Protection Assay With Sandwich Hybridization (NPA-SH) to Monitor Heterosigma akashiwo
Author(s)
강민경; 박미례; 김강은; 이택견
KIOST Author(s)
Kim, Kangeun(김강은)Lee, Taek Kyun(이택견)
Publication Year
2020
Abstract
Heterosigma akashiwo is a globally distributed raphidophyte that forms blooms and causes significant losses to the aquaculture industry in many coastal countries. The development of a fast and sensitive detection method is therefore required to facilitate the appropriate warning of harmful algal blooms. In this study, a nuclease protection integrated with sandwich hybridization (NPA-SH) assay was developed to both qualitatively and quantitatively detect H. akashiwo. The NPA, capture and signal probes were designed by nucleotide sequencing of H. akashiwo. The applicability of NPA-SH was evaluated using cultured H. akashiwo cells and field samples collected at Goseong Bay, Korea. The results show that this method has good applicability and effectiveness in analyzing cultured cells and field samples. A linear regression equation for the quantitative analysis of H. akashiwo was obtained, and the lower detection limit of the assay was 1×104 cells/ml. There was no statistically significant difference in the results of H. akashiwo quantitation using NPA-SH compared to those obtained using a microscope. These results indicate that NPA-SH can be a good alternative to the traditional microscopic method used to monitor H. akashiwo.
ISSN
1225-9918
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38827
Bibliographic Citation
생명과학회지, v.30, no.1, pp.26 - 31, 2020
Publisher
한국생명과학회
Keywords
Algal blooms; field monitoring; HABs; Heterosigma akashiwo; NPA-SH
Type
Article
Language
English
Publisher
한국생명과학회
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