The Effect of Ultrasonificated Extracts of Spirulina maxima on the Anticancer Activity SCIE SCOPUS

Cited 41 time in WEB OF SCIENCE Cited 47 time in Scopus
Title
The Effect of Ultrasonificated Extracts of Spirulina maxima on the Anticancer Activity
Author(s)
Oh, Sung-Ho; Ahn, Juhee; Kang, Do-Hyung; Lee, Hyeon-Yong
KIOST Author(s)
Kang, Do Hyung(강도형)
Publication Year
2011-04
Abstract
The effect of ultrasonic extraction on extraction yields, cytotoxicity, and anticancer activity of Spirulina maxima was investigated in this study. Optimal extraction conditions were determined as 60 kHz frequency at 60A degrees C for 30 min with 120 W intensity, which resulted in 19.3% of extraction yields and 19.1% of cytotoxicity on normal human cells. Yields from conventional water and ethanol extraction were 15.8% at 100A degrees C and 8.3% at 80A degrees C, respectively. It was found that the extracts obtained by ultrasonic extraction process selectively inhibited the digestive-related cancer cell lines, such as human stomach cancer cells, having 89% of the highest inhibition ratio and 4.5 of the highest selectivity. In adding 0.5 mg/mL of the extract, human promyelocytic leukemia cells' cell differentiation was increased 1.72 times over that of the control. Expression level of B cell lymphoma-2 from Hep3B cell was also effectively suppressed by the extract obtained at 60 kHz and 60A degrees C, leading to the inhibition of the early step of carcinogenesis. This work suggests that anticancer activity of the extracts is due to water-soluble polysaccharides rather than proteins and is further supported by the result that the ultrasonification extraction process can efficiently extract relatively intact polysaccharides rather than digesting the proteins in S. maxima by matrix assisted laser desorption ionization-time of flight and high performance size exclusion chromatography chromatogram analyses. Therefore, ultrasonic extraction increases both extraction yield and the biological activity of S. maxima extracts, which might be useful as an alternative natural anticancer agent in the medical and food industries.
ISSN
1436-2228
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3880
DOI
10.1007/s10126-010-9282-2
Bibliographic Citation
MARINE BIOTECHNOLOGY, v.13, no.2, pp.205 - 214, 2011
Publisher
SPRINGER
Subject
GROWTH-PERFORMANCE; BCL-2; DIFFERENTIATION; APOPTOSIS; EXPRESSION; PLATENSIS; CANCER; CELLS
Keywords
Spirulina maxima; Ultrasonification low-temperature extraction; Anticancer activity
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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