Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus SCIE SCOPUS

Cited 2 time in WEB OF SCIENCE Cited 2 time in Scopus
Title
Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicus
Author(s)
Ding, Yuling; Jiratchayamaethasakul, Chanipa; Kim, Junseong; Kim, Eun-A; Heo, Soo-Jin; Lee, Seung-Hong
KIOST Author(s)
Kim, Junseong(김준성)Kim, Eun A(김은아)Heo, Soo Jin(허수진)
Publication Year
2020-01
Abstract
Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island.Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes (HaCaT) to determine its radical scavenging activity and protective effects against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. Anti-melanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by alpha-melanocyte-stimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins (TRP-1, and TRP-2) were examined.Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50: (0.924 +/- 0.035) and (0.327 +/- 0.006) mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPH- induced HaCaT cells, with no cytotoxicity (12.5-400 mu g/mL). The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50: (2.750 +/- 0.006) mg/mL]. On alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber (25-200 mu g/mL) significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2.Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry.
ISSN
2221-1691
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38758
DOI
10.4103/2221-1691.273092
Bibliographic Citation
ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE, v.10, no.1, pp.33 - 41, 2020
Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
Keywords
Anti-melanogenesis; Antioxidant activity; Cosmeceuticals; Red sea cucumber (Stichopus japonicus); Ultrasonic extract
Type
Article
Language
English
Document Type
Article
Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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