Potential Precursor of Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity and Structural Properties of Peptide from Peptic Hydrolysate of Cutlassfish Muscle SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
Potential Precursor of Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity and Structural Properties of Peptide from Peptic Hydrolysate of Cutlassfish Muscle
Author(s)
Kim, H.-S.; Lee, W.; Jayawardena, T.U.; Kang, Nalae; Kang, M.C.; Ko, S.-C.; Lee, J.M.; Yim, M.-J.; Lee, D.-S.; Jeon, Y.-J.
KIOST Author(s)
Kang, Nalae(강나래)
Publication Year
2020-07-02
Abstract
Peptic hydrolysates were prepared by digesting the cutlassfish muscle protein using pepsin for 1, 3, and 6 h, and their inhibitory activity against angiotensin-I converting enzyme (ACE) was studied. The ACE-inhibitory effect of the peptic hydrolysate of cutlassfish muscle generated at the 3 h time point exhibited the strongest activity. After identifying the optimal hydrolysate, the active peptide was isolated by ultrafiltration, gel permeation, and high performance liquid chromatography (HPLC). The resulting purified peptide was characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS/MS) and was identified to be a 496.44 Da pentapeptide (Phe-Ser-Gly-Gly-Glu). The ACE-inhibitory activity of the active peptide exhibited an IC(50)value of 0.033 +/- 0.003 mg/ml. A molecular docking program was used to simulate the interaction between the peptide and ACE, which revealed that the inhibitory effect was mainly due to the hydrogen bonds between ACE and the peptide. Based on the ACE-inhibitory properties and the molecular docking study of the resulting active peptide, we demonstrated an increase in nitric oxide (NO) production in a dose-dependent manner. In conclusion, cutlassfish protein hydrolysate and the resulting active peptide could be used as active ingredients in functional food as anti-hypertensive agents.
ISSN
1049-8850
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/38596
DOI
10.1080/10498850.2020.1773595
Bibliographic Citation
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY, v.29, no.6, pp.544 - 552, 2020
Publisher
TAYLOR & FRANCIS INC
Subject
ENDOTHELIAL-CELLS; PURIFICATION; PROTEIN; IDENTIFICATION; ANTIOXIDANT
Keywords
Cutlassfish; Angiotensin-I converting enzyme (ACE); inhibitory peptide; peptic hydrolysate; hypertension
Type
Article
Language
English
Document Type
Article
Publisher
TAYLOR & FRANCIS INC
Related Researcher
Research Interests

Marine Biotechnology,Bioactivities,Funcational materials,해양생물공학,생리활성,기능성 소재

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