Oricola thermophila sp. nov., a marine bacterium isolated from tidal flat sediment and emended description of the genus Oricola Hameed et al. 2015

Abstract

A Gram-negative, facultatively anaerobic, rod-shaped (1.8-4.4 μm  0.5-0.7 μm) and motile marine bacterium, designated as MEBiC13590T was isolated from tidal flat sediment of the Incheon City, West seaside of Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC13590T showed high similarity with the Oricola cellulosilytica CC-AMH-0T (98.0%) and followed by Oceaniradius stylonematis StC1T (97.2%), however, clustered with Orc. cellulosilytica. Growth was observed at 22-50°C (optimum 45°C), at pH 5-9 (optimum pH 7) and with 1-6% (optimum 3%) NaCl. The predominant cellular fatty acids were C16:0 (7.6%), C18:0 (12.2%), 11-methyl C18:1ω7c (5.7%), C19:0 cycloω6c and summed feature 8 (comprised of C18:1ω7c and/or C18:1ω6c; 38%). The DNA G+C contents is 63.5 mol%. The major respiratory quinone is Q-10. Several phenotypic characteristics such as growth temperature, oxygen requirement, enzyme activities of urease, gelatinase, lipase (C14), α-chymotrypsin, acid phosphatase, β-galactosidase and β-glucosidase etc. differentiate strain MEBiC13590T from Oricola cellulosilytica CC-AMH-0T. Based on this polyphasic taxonomic data, strain MEBiC13590T should be classified as a novel species in the genus Oricola and it is proposed as Oricola thermophila sp. nov. The type strain is MEBiC13590T