Isolation, Purification, and Enzymatic Characterization of Extracellular Chitosanase from Marine Bacterium Bacillus subtilis CH2
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Title
- Isolation, Purification, and Enzymatic Characterization of Extracellular Chitosanase from Marine Bacterium Bacillus subtilis CH2
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Author(s)
- Oh, Chulhong; De Zoysa, Mahanama; Kang, Do-Hyung; Lee, Youngdeuk; Whang, Ilson; Nikapitiya, Chamilani; Heo, Soo-Jin; Yoon, Kon-Tak; Affan, Abu; Lee, Jehee
- KIOST Author(s)
- Oh, Chul Hong(오철홍); Kang, Do Hyung(강도형); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 오철홍; 강도형; 허수진; 윤건탁; Abu Affan
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Publication Year
- 2011-10
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Abstract
- A Bacillus subtilis strain was isolated from the intestine of Sebastiscus marmoratus (scorpion fish) that was identified as Bacillus subtilis CH2 by morphological, biochemical, and genetic analyses. The chitosanase of Bacillus subtilis CH2 was best induced by fructose and not induced with chitosan, unlike other chitosanases. The strain was incubated in LB broth, and the chitosanase secreted into the medium was concentrated with ammonium sulfate precipitation and purified by gel permeation chromatography. The molecular mass of the purified chitosanase was detected as 29 kDa. The optimum pH and temperature of the purified chitosanase were 5.5 and 60 degrees C, respectively. The purified chitosanase was continuously thermostable at 40 degrees C. The specific acitivity of the purified chitosanase was 161 units/mg. The N-terminal amino acid sequence was analyzed for future study.
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ISSN
- 1017-7825
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3806
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DOI
- 10.4014/jmb.1104.04032
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Bibliographic Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.10, pp.1021 - 1025, 2011
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Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
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Subject
- HEXA-N-ACETYLCHITOHEXAOSE; ACETYL CHITOHEXAOSE; ACTION PATTERN; STREPTOMYCES; CHITOOLIGOSACCHARIDES; GROWTH
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Keywords
- Chitosan; chitosanase; chito-oligosaccharide; Bacillus subtilis; Sebastiscus marmoratus; purification
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Type
- Article
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Language
- English
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Document Type
- Article
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