Purification and Biochemical Characterization of Insoluble Acid Invertase (INAC-INV) from Pea Seedlings SCIE SCOPUS

DC Field Value Language
dc.contributor.author Kim, Donggiun -
dc.contributor.author Lee, Gunsup -
dc.contributor.author Chang, Man -
dc.contributor.author Park, Jongbum -
dc.contributor.author Chung, Youngjae -
dc.contributor.author Lee, Sukchan -
dc.contributor.author Lee, Taek-Kyun -
dc.date.accessioned 2020-04-20T07:43:18Z -
dc.date.available 2020-04-20T07:43:18Z -
dc.date.created 2020-01-28 -
dc.date.issued 2011-10-26 -
dc.identifier.issn 0021-8561 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3801 -
dc.description.abstract Invertase (EC 3.2.1.26) catalyzes the hydrolysis of sucrose into D-glucose and D-fructose. Insoluble acid invertase (INAC-INV) was purified from pea (Pisum sativum L.) by sequential procedures entailing ammonium sulfate precipitation, ion exchange chromatography, absorption chromatography, reactive green-19 affinity chromatography, and gel filtration. The purified INAC-INV had a pH optimum of 4.0 and a temperature optimum of 45 degrees C. The effects of various concentrations of Tris-HCl, HgCl2, and CuSO4 on the activities of the purified invertase were examined. INAC-INV was not affected by Tris-HCl and HgCl2. INAC-INV activity was inhibited by 6.2 mM CuSO4 up to 50%. The enzymes display typical hyperbolic saturation kinetics for sucrose hydrolysis. The K-m and V-max values of INAC-INV were determined to be 4.41 mM and 8.41 U (mg protein)(-1) min(-1), respectively. INAC-INV is a true member of the beta-fructofuranosidases, which can react with sucrose and raffinose as substrates. SDS-PAGE and immunoblotting were used to determine the molecular mass of INAC-INV to be 69 kDa. The isoelectric point of INAC-INV was estimated to be about pH 8.0. Taken together, INAC-INV is a pea seedling invertase with a stable and optimum activity at lower acid pH and at higher temperature than other invertases. -
dc.description.uri 1 -
dc.language English -
dc.publisher AMER CHEMICAL SOC -
dc.subject CELL-WALL INVERTASE -
dc.subject BETA-FRUCTOSIDASE -
dc.subject ALKALINE INVERTASE -
dc.subject SOLUBLE INVERTASES -
dc.subject RADISH SEEDLINGS -
dc.subject FRUCTOFURANOSIDASE -
dc.subject CARROT -
dc.subject LEAVES -
dc.subject EXPRESSION -
dc.subject INFECTION -
dc.title Purification and Biochemical Characterization of Insoluble Acid Invertase (INAC-INV) from Pea Seedlings -
dc.type Article -
dc.citation.endPage 11233 -
dc.citation.startPage 11228 -
dc.citation.title JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY -
dc.citation.volume 59 -
dc.citation.number 20 -
dc.contributor.alternativeName 이건섭 -
dc.contributor.alternativeName 장만 -
dc.contributor.alternativeName 이택견 -
dc.identifier.bibliographicCitation JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.59, no.20, pp.11228 - 11233 -
dc.identifier.doi 10.1021/jf201057c -
dc.identifier.scopusid 2-s2.0-80055014023 -
dc.identifier.wosid 000295915000056 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus CELL-WALL INVERTASE -
dc.subject.keywordPlus BETA-FRUCTOSIDASE -
dc.subject.keywordPlus ALKALINE INVERTASE -
dc.subject.keywordPlus SOLUBLE INVERTASES -
dc.subject.keywordPlus RADISH SEEDLINGS -
dc.subject.keywordPlus FRUCTOFURANOSIDASE -
dc.subject.keywordPlus CARROT -
dc.subject.keywordPlus LEAVES -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus INFECTION -
dc.subject.keywordAuthor insoluble acid invertase -
dc.subject.keywordAuthor purification -
dc.subject.keywordAuthor characterization -
dc.subject.keywordAuthor Pisum sativum L. -
dc.relation.journalWebOfScienceCategory Agriculture, Multidisciplinary -
dc.relation.journalWebOfScienceCategory Chemistry, Applied -
dc.relation.journalWebOfScienceCategory Food Science & Technology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Agriculture -
dc.relation.journalResearchArea Chemistry -
dc.relation.journalResearchArea Food Science & Technology -
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