Purification and Biochemical Characterization of Insoluble Acid Invertase (INAC-INV) from Pea Seedlings SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Kim, Donggiun | - |
dc.contributor.author | Lee, Gunsup | - |
dc.contributor.author | Chang, Man | - |
dc.contributor.author | Park, Jongbum | - |
dc.contributor.author | Chung, Youngjae | - |
dc.contributor.author | Lee, Sukchan | - |
dc.contributor.author | Lee, Taek-Kyun | - |
dc.date.accessioned | 2020-04-20T07:43:18Z | - |
dc.date.available | 2020-04-20T07:43:18Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2011-10-26 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/3801 | - |
dc.description.abstract | Invertase (EC 3.2.1.26) catalyzes the hydrolysis of sucrose into D-glucose and D-fructose. Insoluble acid invertase (INAC-INV) was purified from pea (Pisum sativum L.) by sequential procedures entailing ammonium sulfate precipitation, ion exchange chromatography, absorption chromatography, reactive green-19 affinity chromatography, and gel filtration. The purified INAC-INV had a pH optimum of 4.0 and a temperature optimum of 45 degrees C. The effects of various concentrations of Tris-HCl, HgCl2, and CuSO4 on the activities of the purified invertase were examined. INAC-INV was not affected by Tris-HCl and HgCl2. INAC-INV activity was inhibited by 6.2 mM CuSO4 up to 50%. The enzymes display typical hyperbolic saturation kinetics for sucrose hydrolysis. The K-m and V-max values of INAC-INV were determined to be 4.41 mM and 8.41 U (mg protein)(-1) min(-1), respectively. INAC-INV is a true member of the beta-fructofuranosidases, which can react with sucrose and raffinose as substrates. SDS-PAGE and immunoblotting were used to determine the molecular mass of INAC-INV to be 69 kDa. The isoelectric point of INAC-INV was estimated to be about pH 8.0. Taken together, INAC-INV is a pea seedling invertase with a stable and optimum activity at lower acid pH and at higher temperature than other invertases. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CELL-WALL INVERTASE | - |
dc.subject | BETA-FRUCTOSIDASE | - |
dc.subject | ALKALINE INVERTASE | - |
dc.subject | SOLUBLE INVERTASES | - |
dc.subject | RADISH SEEDLINGS | - |
dc.subject | FRUCTOFURANOSIDASE | - |
dc.subject | CARROT | - |
dc.subject | LEAVES | - |
dc.subject | EXPRESSION | - |
dc.subject | INFECTION | - |
dc.title | Purification and Biochemical Characterization of Insoluble Acid Invertase (INAC-INV) from Pea Seedlings | - |
dc.type | Article | - |
dc.citation.endPage | 11233 | - |
dc.citation.startPage | 11228 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 59 | - |
dc.citation.number | 20 | - |
dc.contributor.alternativeName | 이건섭 | - |
dc.contributor.alternativeName | 장만 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.59, no.20, pp.11228 - 11233 | - |
dc.identifier.doi | 10.1021/jf201057c | - |
dc.identifier.scopusid | 2-s2.0-80055014023 | - |
dc.identifier.wosid | 000295915000056 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CELL-WALL INVERTASE | - |
dc.subject.keywordPlus | BETA-FRUCTOSIDASE | - |
dc.subject.keywordPlus | ALKALINE INVERTASE | - |
dc.subject.keywordPlus | SOLUBLE INVERTASES | - |
dc.subject.keywordPlus | RADISH SEEDLINGS | - |
dc.subject.keywordPlus | FRUCTOFURANOSIDASE | - |
dc.subject.keywordPlus | CARROT | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordAuthor | insoluble acid invertase | - |
dc.subject.keywordAuthor | purification | - |
dc.subject.keywordAuthor | characterization | - |
dc.subject.keywordAuthor | Pisum sativum L. | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |