Characterization of a novel molluscan MyD88 family protein from manila clam, Ruditapes philippinarum SCIE SCOPUS

Cited 30 time in WEB OF SCIENCE Cited 32 time in Scopus
Title
Characterization of a novel molluscan MyD88 family protein from manila clam, Ruditapes philippinarum
Author(s)
Lee, Youngdeuk; Whang, Ilson; Umasuthan, Navaneethaiyer; De Zoysa, Mahanama; Oh, Chulhong; Kang, Do-Hyung; Choi, Cheol Young; Park, Choul-Ji; Lee, Jehee
KIOST Author(s)
Oh, Chul Hong(오철홍)Kang, Do Hyung(강도형)
Publication Year
2011-12
Abstract
Myeloid differentiation factor 88 (MyD88) is a universal adaptor protein which is required for signal transduction of TLR/IL-1R family. In this study, a novel molluscan MyD88 family member protein (named as RpMyD88) was identified from manila clam, Ruditapes philippinarum. It was identified using BLAST algorithm from GS-FLX (TM) sequencing data. The cDNA of RpMyD88 consists of 1416 bp open reading frame (ORF) encoding 471 amino acid residues. The RpMyD88 contains death domain and Toll/interleukin-1 receptor (TIR) domain which are typical features of MyD88 family proteins. The predicted amino acid sequence of RpMyD88 shares 27% identity with scallop MyD88. The expression level of RpMyD88 mRNA was investigated in healthy and challenged clams by quantitative real-time RT-PCR. The RpMyD88 gene expression is ubiquitous in all selected tissues. The RpMyD88 mRNA was strongly expressed in hemocyte, gill and mantle. In contrast, it was weakly expressed in siphon, foot and adductor muscle. RpMyD88 was up-regulated in gill and hemocyte after immune challenge with both Vibrio tapetis and LPS challenge. All results considered, sequence characterization, comparison and gene expression data suggesting that MyD88-dependent signaling pathway is presence in manila clam and RpMyD88 plays an important role in innate immune response against bacteria. (C) 2011 Elsevier Ltd. All rights reserved.
ISSN
1050-4648
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3787
DOI
10.1016/j.fsi.2011.08.003
Bibliographic Citation
FISH & SHELLFISH IMMUNOLOGY, v.31, no.6, pp.887 - 893, 2011
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Subject
TOLL-LIKE-RECEPTORS; SIGNALING PATHWAYS; ADAPTER PROTEIN; INNATE IMMUNITY; CDNA; RECOGNITION; CLONING; GENE; IDENTIFICATION; EXPRESSION
Keywords
Manila clam; Ruditapes philippinarum; Mollusc; Invertebrate; Myeloid differentiation factor 88 (MyD88)
Type
Article
Language
English
Document Type
Article
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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