Effect of culture density and antioxidants on naupliar production and gene expression of the cyclopoid copepod, Paracyclopina nana SCIE SCOPUS

Cited 40 time in WEB OF SCIENCE Cited 41 time in Scopus
Title
Effect of culture density and antioxidants on naupliar production and gene expression of the cyclopoid copepod, Paracyclopina nana
Author(s)
Lee, Kyun-Woo; Rhee, Jae-Sung; Han, Jeonghoon; Park, Heum Gi; Lee, Jae-Seong
KIOST Author(s)
Lee, Kyun Woo(이균우)
Publication Year
2012-02
Abstract
Although attempts have been made to use mass cultures of marine copepods as live foods in marine aquaculture, some limitations such as low density culture still exist. The brackish water cyclopoid copepod, Paracyclopina nana has the potential for mass culturing as live food. In this study, we not only investigated the effect of culture density on the naupliar production and specific gene expressions of P. nana, but also the effect of several antioxidants under the conditions of a high density culture. The naupliar production of the copepod decreased with increasing culture density. The expression of glutathione reductase (GR), selenium-dependent glutathione peroxidase (SeGPx), glutathione S-transferase kappa (GST kappa), heat shock protein 40 (Hsp40), and Hsp70 genes of P. nano increased in the high density treatment but vitellogenin genes (Vg1 and Vg2) showed downregulation. In the condition with 20 inds./mL vitamin C had a significant decrease but sodium selenite induced the naupliar production of P. nana greatly. The expressions of GR, SeGPx, Hsp70, and Vg genes increased with the vitamin C treatment. Sodium selenite caused a decrease of SeGPx and Hsp40 but GST kappa increased in the treatment with 20 inds./mL These results suggest that sodium selenite is a positive antioxidant which can increase the culture efficiency of the copepod. (C) 2011 Elsevier Inc. All rights reserved.
ISSN
1095-6433
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3637
DOI
10.1016/j.cbpa.2011.10.019
Bibliographic Citation
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v.161, no.2, pp.145 - 152, 2012
Publisher
ELSEVIER SCIENCE INC
Subject
GLUTATHIONE-PEROXIDASE ACTIVITY; TROUT ONCORHYNCHUS-MYKISS; HEAT-SHOCK PROTEINS; TIGRIOPUS-JAPONICUS; INTERTIDAL COPEPOD; MARINE COPEPOD; POPULATION-GROWTH; STOCKING DENSITY; SODIUM SELENITE; EGG-PRODUCTION
Keywords
Paracyclopina nana; Culture density; Antioxidant; Gene expression
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCIENCE INC
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