Neuroprotective Effect of Phlorotannin Isolated from Ishige okamurae Against H2O2-Induced Oxidative Stress in Murine Hippocampal Neuronal Cells, HT22
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Title
- Neuroprotective Effect of Phlorotannin Isolated from Ishige okamurae Against H2O2-Induced Oxidative Stress in Murine Hippocampal Neuronal Cells, HT22
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Author(s)
- Heo, Soo-Jin; Cha, Seon-Heui; Kim, Kil-Nam; Lee, Seung-Hong; Ahn, Ginnae; Kang, Do-Hyung; Oh, Chulhong; Choi, Young-Ung; Affan, Abu; Kim, Daekyung; Jeon, You-Jin
- KIOST Author(s)
- Heo, Soo Jin(허수진); Kang, Do Hyung(강도형); Oh, Chul Hong(오철홍); Choi, Young Ung(최영웅)
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Alternative Author(s)
- 허수진; 강도형; 오철홍; 최영웅; Abu Affan
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Publication Year
- 2012-03
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Abstract
- The present study is designed to investigate the neuroprotective effect of a kind of phlorotannins, diphlorethohydroxycarmalol (DPHC) isolated from Ishige okamurae against hydrogen peroxide (H2O2)-induced oxidative stress in murine hippocampal neuronal cells, HT22. H2O2 treatment induced neurotoxicity, whereas DPHC prevented cells from H2O2-induced damage then restoring cell viability was significantly increased. DPHC slightly reduced the expression of Bax induced by H2O2 but recovered the expression of Bcl-xL as well as caspase-9 and -3 mediated PARP cleavage by H2O2. Intracellular reactive oxygen species (ROS) and lipid peroxidation was overproduced as the result of the addition of H2O2; however, these ROS generations and lipid peroxidation were effectively inhibited by addition of DPHC in a dose-dependent manner. Moreover, DPHC suppressed the elevation of H2O2-induced Ca2+ release. These findings indicate that DPHC has neuroprotective effects against H2O2-induced damage in neuronal cells, and that an inhibitory effect on ROS production may contribute to the underlying mechanisms.
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ISSN
- 0273-2289
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3622
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DOI
- 10.1007/s12010-012-9545-7
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Bibliographic Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.166, no.6, pp.1520 - 1532, 2012
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Publisher
- HUMANA PRESS INC
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Subject
- GLUTAMATE; DEATH; DAMAGE; APOPTOSIS; ACTIVATION; TOXICITY; ASSAY; LINE; DIPHLORETHOHYDROXYCARMALOL; ANTIOXIDANT
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Keywords
- Neuroprotection; HT22; Oxidative stress; Hydrogen peroxide; Ishige okamurae
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Type
- Article
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Language
- English
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Document Type
- Article
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