Neuroprotective Effect of Phlorotannin Isolated from Ishige okamurae Against H2O2-Induced Oxidative Stress in Murine Hippocampal Neuronal Cells, HT22 SCIE SCOPUS

Cited 36 time in WEB OF SCIENCE Cited 35 time in Scopus
Title
Neuroprotective Effect of Phlorotannin Isolated from Ishige okamurae Against H2O2-Induced Oxidative Stress in Murine Hippocampal Neuronal Cells, HT22
Author(s)
Heo, Soo-Jin; Cha, Seon-Heui; Kim, Kil-Nam; Lee, Seung-Hong; Ahn, Ginnae; Kang, Do-Hyung; Oh, Chulhong; Choi, Young-Ung; Affan, Abu; Kim, Daekyung; Jeon, You-Jin
KIOST Author(s)
Heo, Soo Jin(허수진)Kang, Do Hyung(강도형)Oh, Chul Hong(오철홍)
Publication Year
2012-03
Abstract
The present study is designed to investigate the neuroprotective effect of a kind of phlorotannins, diphlorethohydroxycarmalol (DPHC) isolated from Ishige okamurae against hydrogen peroxide (H2O2)-induced oxidative stress in murine hippocampal neuronal cells, HT22. H2O2 treatment induced neurotoxicity, whereas DPHC prevented cells from H2O2-induced damage then restoring cell viability was significantly increased. DPHC slightly reduced the expression of Bax induced by H2O2 but recovered the expression of Bcl-xL as well as caspase-9 and -3 mediated PARP cleavage by H2O2. Intracellular reactive oxygen species (ROS) and lipid peroxidation was overproduced as the result of the addition of H2O2; however, these ROS generations and lipid peroxidation were effectively inhibited by addition of DPHC in a dose-dependent manner. Moreover, DPHC suppressed the elevation of H2O2-induced Ca2+ release. These findings indicate that DPHC has neuroprotective effects against H2O2-induced damage in neuronal cells, and that an inhibitory effect on ROS production may contribute to the underlying mechanisms.
ISSN
0273-2289
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3622
DOI
10.1007/s12010-012-9545-7
Bibliographic Citation
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.166, no.6, pp.1520 - 1532, 2012
Publisher
HUMANA PRESS INC
Subject
GLUTAMATE; DEATH; DAMAGE; APOPTOSIS; ACTIVATION; TOXICITY; ASSAY; LINE; DIPHLORETHOHYDROXYCARMALOL; ANTIOXIDANT
Keywords
Neuroprotection; HT22; Oxidative stress; Hydrogen peroxide; Ishige okamurae
Type
Article
Language
English
Document Type
Article
Publisher
HUMANA PRESS INC
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