Isolation and Characterization of a Lytic Myoviridae Bacteriophage PAS-1 with Broad Infectivity in Aeromonas salmonicida SCIE SCOPUS

Cited 25 time in WEB OF SCIENCE Cited 27 time in Scopus
Title
Isolation and Characterization of a Lytic Myoviridae Bacteriophage PAS-1 with Broad Infectivity in Aeromonas salmonicida
Author(s)
Kim, J. H.; Son, J. S.; Choi, Y. J.; Choresca, C. H.; Shin, S. P.; Han, J. E.; Jun, J. W.; Kang, D. H.; Oh, C.; Heo, S. J.; Park, S. C.
KIOST Author(s)
Kang, Do-Hyung(강도형)Oh, Chulhong(오철홍)Heo, Soo Jin(허수진)
Publication Year
2012-05
Abstract
To search for candidate control agents against Aeromonas salmonicida subsp. salmonicida infections in aquaculture, one bacteriophage (phage), designated as PAS-1, was isolated from the sediment samples of the rainbow trout (Oncorhynchus mykiss) culture farm in Korea. The PAS-1 was morphologically classified as Myoviridae and possessed approximately 48 kb of double-strand genomic DNA. The phage showed broad host ranges to other subspecies of A. salmonicida as well as A. salmonicida subsp. salmonicida including antibiotic-resistant strains. Its latent period and burst size were estimated to be approximately 40 min and 116.7 PFU/cell, respectively. Furthermore, genomic and structural proteomic analysis of PAS-1 revealed that the phage was closely related to other Myoviridae phages infecting enterobacteria or Aeromonas species. The bacteriolytic activity of phage PAS-1 was evaluated using three subspecies of A. salmonicida strain at different doses of multiplicity of infection, and the results proved to be efficient for the reduction of bacterial growth. Based on these results, PAS-1 could be considered as a novel Aeromonas phage and might have potentiality to reduce the impacts of A. salmonicida infections in aquaculture.
ISSN
0343-8651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3591
DOI
10.1007/s00284-012-0091-x
Bibliographic Citation
CURRENT MICROBIOLOGY, v.64, no.5, pp.418 - 426, 2012
Publisher
SPRINGER
Subject
ENTEROCOCCUS-FAECALIS; VIBRIO-HARVEYI; PHAGES; FURUNCULOSIS; BIOCONTROL; POLYMERASE; SEQUENCE; THERAPY; GENOME; GENE
Keywords
Aeromonas salmonicida; Bacteriophage; PAS-1; Myoviridae; Aquaculture
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
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