Proteome Analyses of Hydrogen-producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1 in Different One-carbon Substrate Culture Conditions SCIE SCOPUS

Cited 28 time in WEB OF SCIENCE Cited 28 time in Scopus
Title
Proteome Analyses of Hydrogen-producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1 in Different One-carbon Substrate Culture Conditions
Author(s)
Moon, Yoon-Jung; Kwon, Joseph; Yun, Sung-Ho; Lim, Hye Li; Kim, Min-Sik; Kang, Sung Gyun; Lee, Jung-Hyun; Choi, Jong-Soon; Kim, Seung Il; Chung, Young-Ho
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Jung Hyun(이정현)
Publication Year
2012-06
Abstract
Thermococcus onnurineus NA1, a sulfur-reducing hyperthermophilic archaeon, is capable of H-2-producing growth, considered to be hydrogenogenic carboxydotrophy. Utilization of formate as a sole energy source has been well studied in T. onnurineus NA1. However, whether formate can be used as its carbon source remains unknown. To obtain a global view of the metabolic characteristics of H-2-producing growth, a quantitative proteome analysis of T. onnurineus NA1 grown on formate, CO, and starch was performed by combining one-dimensional SDS-PAGE with nano UPLC-MSE. A total of 587 proteins corresponding to 29.7% of the encoding genes were identified, and the major metabolic pathways (especially energy metabolism) were characterized at the protein level. Expression of glycolytic enzymes was common but more highly induced in starch-grown cells. In contrast, enzymes involved in key steps of the gluconeogenesis and pentose phosphate pathways were strongly up-regulated in formate-grown cells, suggesting that formate could be utilized as a carbon source by T. onnurineus NA1. In accordance with the genomic analysis, comprehensive proteomic analysis also revealed a number of hydrogenase clusters apparently associated with formate metabolism. On the other hand, CODH and CO-induced hydrogenases belonging to the Hyg4-II cluster, as well as sulfhydrogenase-I and Mbx, were prominently expressed during CO culture. Our data suggest that CO can be utilized as a sole energy source for H-2 production via an electron transport mechanism and that CO2 produced from catabolism or CO oxidation by CODH and CO-induced hydrogenases may subsequently be assimilated into the organic carbon. Overall, proteomic comparison of formate-and CO-grown cells with starch-grown cells revealed that a single carbon compound, such as formate and CO, can be utilized as an efficient substrate to provide cellular carbon and/or energy by T. onnurineus NA1. Molecular & Cellular Proteomics 11: 10.1074/mcp.M111.015420, 1-15, 2012.
ISSN
1535-9476
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3587
DOI
10.1074/mcp.M111.015420
Bibliographic Citation
MOLECULAR & CELLULAR PROTEOMICS, v.11, no.6, 2012
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Subject
PYRUVATE-FERREDOXIN OXIDOREDUCTASE; COMPLETE GENOME SEQUENCE; SEA HYDROTHERMAL VENT; RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE; PYROCOCCUS-FURIOSUS; H-2 PRODUCTION; CO OXIDATION; CARBOXYDOTHERMUS HYDROGENOFORMANS; METHANOBACTERIUM-FORMICICUM; DEOXYRIBONUCLEIC ACID
Type
Article
Language
English
Document Type
Article
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse