Molecular characterization, expression, and functional analysis of two thioredoxins in the black rockfish (Sebastes schlegelii)
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Title
- Molecular characterization, expression, and functional analysis of two thioredoxins in the black rockfish (Sebastes schlegelii)
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Author(s)
- Park, Chan-Il; Jung, Jee-Hyun; Shim, Won Joon; Kim, Ju-Won; Kim, Eun-Gyeong; Jeong, Ji-Min; Kim, Do-Hyung
- KIOST Author(s)
- Jung, Jee Hyun(정지현); Shim, Won Joon(심원준)
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Alternative Author(s)
- 정지현; 심원준
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Publication Year
- 2012-05
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Abstract
- Thioredoxins (TRxs) are a family of small evolutionarily conserved proteins that are essential for the maintenance of cellular homeostasis. Two TRx homologue cDNAs were isolated from a black rockfish concanavalin A (Con A)/phorbol myristate acetate (PMA)-stimulated leucocyte cDNA library and named BrTPx1-1 and BrTPx1-2. As compared with other known TRx peptide sequences, the most conserved regions of both BrTRx1-1 and BrTRx1-2 peptides were found to be the redox-active site Trp-Cys-X-X-Cys (WCX(C). The TRx present in most species is a TRx1-2 protein with a Cys-Pro-Gly-Cys (CPGC) active site. However, in the larger 13 kDa BrTRx1-1 protein, a Cys-Pro-Pro-Cys (CPPC) active site was identified. Here, we report the identification of a new member of the TRx protein family from the teleost black rockfish, which defines a new subclass of 13-kDa TRx1-1 proteins. Phylogenetic analysis indicated that both BrTRx1-1 and BrTRx1-2 were grouped with other vertebrate TRx1 peptides. BrTRx1-1 expression was strongly induced in peripheral blood leucocytes (PBLs) 12-24 h following Con A/PMA stimulation, with peak expression at 24 h post-stimulation. BrTRx1-2 was induced in PBLs after stimulation with lipopolysaccharide (LPS), Con A/PMA, or poly I:C at 24 h. The BrTRx1-1 gene was predominantly expressed in the liver and gills, while BrTRx1-2 was expressed in PBLs and gills. After treatment with a high concentration (10 mu g/mL) of rBrTRx1-1 or rBrTRx1-2, kidney leucocytes exhibited increased cell proliferation and viability under oxidative stress. (C) 2012 Elsevier Ltd. All rights reserved.
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ISSN
- 1050-4648
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/3562
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DOI
- 10.1016/j.fsi.2012.02.002
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Bibliographic Citation
- FISH & SHELLFISH IMMUNOLOGY, v.32, no.5, pp.808 - 815, 2012
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Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Subject
- LEUKEMIA-DERIVED FACTOR; THIOL-SPECIFIC ANTIOXIDANT; REDOX REGULATION; GROWTH-FACTOR; CELL-GROWTH; PROTEIN; REDUCTASE; CLONING; ACTIVATION; SECRETION
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Keywords
- Antioxidant activity; Black rockfish; MTT; H2O2; Thioredoxin
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Type
- Article
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Language
- English
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Document Type
- Article
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