Crucial Role of TSC-22 in Preventing the Proteasomal Degradation of p53 in Cervical Cancer SCIE SCOPUS

DC Field Value Language
dc.contributor.author Yoon, Cheol-Hee -
dc.contributor.author Rho, Seung Bae -
dc.contributor.author Kim, Seong-Tae -
dc.contributor.author Kho, Seongho -
dc.contributor.author Park, Junsoo -
dc.contributor.author Jang, Ik-Soon -
dc.contributor.author Woo, Seonock -
dc.contributor.author Kim, Sung Soon -
dc.contributor.author Lee, Je-Ho -
dc.contributor.author Lee, Seung-Hoon -
dc.date.accessioned 2020-04-20T06:51:50Z -
dc.date.available 2020-04-20T06:51:50Z -
dc.date.created 2020-01-28 -
dc.date.issued 2012-08-01 -
dc.identifier.issn 1932-6203 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/3480 -
dc.description.abstract The p53 tumor suppressor function can be compromised in many tumors by the cellular antagonist HDM2 and human papillomavirus oncogene E6 that induce p53 degradation. Restoration of p53 activity has strong therapeutic potential. Here, we identified TSC-22 as a novel p53-interacting protein and show its novel function as a positive regulator of p53. We found that TSC-22 level was significantly down-regulated in cervical cancer tissues. Moreover, over-expression of TSC-22 was sufficient to inhibit cell proliferation, promote cellular apoptosis in cervical cancer cells and suppress growth of xenograft tumors in mice. Expression of also TSC-22 enhanced the protein level of p53 by protecting it from poly-ubiquitination. When bound to the motif between amino acids 100 and 200 of p53, TSC-22 inhibited the HDM2- and E6-mediated p53 polyubiquitination and degradation. Consequently, ectopic over-expression of TSC-22 activated the function of p53, followed by increased expression of p21(Waf1/Cip1) and PUMA in human cervical cancer cell lines. Interestingly, TSC-22 did not affect the interaction between p53 and HDM2. Knock-down of TSC-22 by small interfering RNA clearly enhanced the polyubiquitination of p53, leading to the degradation of p53. These results suggest that TSC-22 acts as a tumor suppressor by safeguarding p53 from poly-ubiquitination mediated-degradation. -
dc.description.uri 1 -
dc.language English -
dc.publisher PUBLIC LIBRARY SCIENCE -
dc.subject BETA-STIMULATED CLONE-22 -
dc.subject SALIVARY-GLAND CANCER -
dc.subject GROWTH-FACTOR-BETA -
dc.subject DOWN-REGULATION -
dc.subject CELL LINE -
dc.subject IN-VIVO -
dc.subject MDM2 -
dc.subject SUPPRESSOR -
dc.subject GENE -
dc.subject PROTEIN -
dc.title Crucial Role of TSC-22 in Preventing the Proteasomal Degradation of p53 in Cervical Cancer -
dc.type Article -
dc.citation.title PLOS ONE -
dc.citation.volume 7 -
dc.citation.number 8 -
dc.contributor.alternativeName 우선옥 -
dc.identifier.bibliographicCitation PLOS ONE, v.7, no.8 -
dc.identifier.doi 10.1371/journal.pone.0042006 -
dc.identifier.scopusid 2-s2.0-84864764952 -
dc.identifier.wosid 000307212800051 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus BETA-STIMULATED CLONE-22 -
dc.subject.keywordPlus SALIVARY-GLAND CANCER -
dc.subject.keywordPlus GROWTH-FACTOR-BETA -
dc.subject.keywordPlus DOWN-REGULATION -
dc.subject.keywordPlus CELL LINE -
dc.subject.keywordPlus IN-VIVO -
dc.subject.keywordPlus MDM2 -
dc.subject.keywordPlus SUPPRESSOR -
dc.subject.keywordPlus GENE -
dc.subject.keywordPlus PROTEIN -
dc.relation.journalWebOfScienceCategory Multidisciplinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Science & Technology - Other Topics -
Appears in Collections:
Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 1. Journal Articles
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