Identification of unique cis-element pattern on simulated microgravity treated Arabidopsis by in silico and gene expression SCIE SCOPUS

Cited 5 time in WEB OF SCIENCE Cited 5 time in Scopus
Title
Identification of unique cis-element pattern on simulated microgravity treated Arabidopsis by in silico and gene expression
Author(s)
Soh, Hyuncheol; Choi, Yongsang; Lee, Taek-Kyun; Yeo, Up-Dong; Han, Kyeongsik; Auh, Chungkyun; Lee, Sukchan
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2012-08-01
Abstract
Arabidopsis gene expression microarray (44 K) was used to detect genes highly induced under simulated microgravity stress (SMS). Ten SMS-inducible genes were selected from the microarray data and these 10 genes were found to be abundantly expressed in 3-week-old plants. Nine out of the 10 SMS-inducible genes were also expressed in response to the three abiotic stresses of drought, touch, and wounding in 3-week-old Arabidopsis plants respectively. However, WRKY46 was elevated only in response to SMS. Six other WRKY genes did not respond to SMS. To clarify the characteristics of the genes expressed at high levels in response to SMS, 20 cis-elements in the promoters of the 40 selected genes including the 10 SMS-inducible genes, the 6 WRKY genes, and abiotic stress-inducible genes were analyzed and their spatial positions on each promoter were determined. Four cis-elements (M/T-G-T-P from MYBIAT or TATABOX5, GTICONSENSUS, TATABOX5, and POLASIG1) showed a unique spatial arrangement in most SMS-inducible genes including WRKY46. Therefore the M/T-G-T-P cis-element patterns identified in the promoter of WRKY46 may play important roles in regulating gene expression in response to SMS. The presences of the cis-element patterns suggest that the order or spatial positioning of certain groups of cis-elements is more important than the existence or numbers of specific cis-elements. Taken together, our data indicate that WRKY46 is a novel SMS inducible transcription factor and the unique spatial arrangement of cis-elements shown in WRKY46 promoter may play an important role for its response to SMS. (c) 2012 COSPAR. Published by Elsevier Ltd. All rights reserved.
ISSN
0273-1177
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/3479
DOI
10.1016/j.asr.2012.04.023
Bibliographic Citation
ADVANCES IN SPACE RESEARCH, v.50, no.3, pp.397 - 407, 2012
Publisher
ELSEVIER SCI LTD
Subject
STRESS-RESPONSIVE TRANSCRIPTION; THALIANA CELL-CULTURES; SHORT-TERM EXPOSURE; PLANT DEFENSE; SUPERFAMILY; CALMODULIN; METABOLISM; MICROARRAY; PREDICTION; PROMOTERS
Keywords
Arabidopsis; 3-D clinorotation; In silico analysis; Simulated microgravity stress; Transcription factor; WRKY
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse